TAP光敏剂与乳腺癌细胞DNA的相互作用研究

Study on the Interaction Between TAP Photosensitizer and Breast Cancer Cell DNA

关键词:  光敏剂  肿瘤细胞DNA  单线态氧  乳腺癌  相互作用

Key words:  photosensitizer  tumor cell DNA  singlet oxygen  breast cancer  interaction

摘要:

目的 探索TAP光敏剂[2，4，6-三(二甲氨甲基)苯酚酞菁锌][ZnPc(TAP)]与乳腺癌细胞DNA的结合及其降解作用。方法 探针法检测TAP单线态氧的产量及其类型；紫外光谱法研究TAP光敏剂与乳腺癌细胞DNA的相互作用并计算其结合常数；通过凝胶电泳，成像和核酸检测等方法在细胞内、外探索并分析TAP光敏剂对乳腺癌细胞DNA的降解作用。结果 TAP光敏剂具有良好的紫外吸收和荧光光谱性能，其单线态氧产量丰富，能够产生显著的氢氧自由基实现光动力作用。TAP光敏剂与乳腺癌肿瘤细胞DNA存在强相互作用，其结合常数(Kdu)为3.72×10⁶。细胞内、外光动力试验均证明TAP光敏剂能够降解乳腺癌肿瘤细胞DNA，其降解作用与光敏剂浓度呈正相关。结论 TAP光敏剂与乳腺癌细胞DNA结合并产生显著的光动力降解作用，有助于推动TAP光敏剂在肿瘤DNA方面的应用，为乳腺癌的转移和复发治疗提供参考价值。

Abstract:

OBJECTIVE To objective the binding and degradation of TAP photosensitizer[ZnPc(TAP)] with breast cancer cell DNA. METHODS TAP singlet oxygen production and its types were detected by probe method. The interaction between TAP photosensitizer and DNA of breast cancer cells was studied by UV spectroscopy and its binding constants were calculated. The degradation of TAP on DNA of breast cancer cells was explored and analyzed intracellular and extracellular by gel electrophoresis, imaging and nucleic acid detection. RESULTS It was proved that TAP photosensitizer had good UV absorption and fluorescence spectrum properties, Its production of singlet oxygen was abundant. It could produce significant hydrogen and oxygen radicals to achieve photodynamic effect. TAP photosensitizer had strong interaction with breast cancer cell line DNA with a binding constant(Kdu) as 3.72×10⁶. Both intracellular and extracellular photodynamic experiments demonstrated that TAP photosensitizer could degrade DNA in breast cancer cell line, and its degradation was positively correlated with the concentration of photosensitizer. CONCLUSION TAP photosensitizer has significant photodynamic degradation effect on breast cancer cell DNA, which is helpful to promote the application of TAP photosensitizer in cancer DNA and provide reference value for the treatment of breast cancer metastasis and recurrence.