HPLC同时测定大青叶中2个专属性酚酸的含量

Simultaneous Determination of 2 Specific Phenolic Acid in Isatidis Folium by HPLC

关键词:  大青叶  高效液相色谱法  酚酸类  含量测定

Key words:  Isatidis Folium  HPLC  phenolic acids  content determination

摘要:

目的 建立同时测定大青叶中1,2,2'-tri-O-E-sinapoyl-β-gentiobiose和1,2,6'-tri-O-E-sinapoylgentiobiose 2个专属性酚酸的含量方法。方法 采用依利特C₁₈色谱柱(4.6 mm×250 mm，5 μm)，以乙腈-0.1%磷酸溶液为流动相，梯度洗脱，流速为1.0 mL·min^-1，检测波长328 nm，柱温30 ℃。结果 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose和1,2,6'-tri-O-E- sinapoylgentiobiose分别在2.092~104.6 μg·mL^-1(R²=0.999 6)和2.996~149.8 μg·mL^-1(R²=0.999 5)内呈现出良好的线性关系；精密度、稳定性和重复性的RSD均<2%；平均加样回收率(n=6)分别为99.05%，99.60%，RSD分别为0.34%，0.65%。结论 该方法简便，专属性、重复性好，可用于测定大青叶2个专属性酚酸的含量测定方法。

Abstract:

OBJECTIVE To establish a method for the simultaneous determination of 2 specific phenolic acids glycosides 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose in Isatidis Folium. METHODS The HPLC analysis was carried on Elite C₁₈ column(4.6 mm×250 mm, 5 μm) with mobile phase consisted of acetonitrile-0.1% phosphoric acid aqueous solution(gradient elution) at the flow rate of 1.0 mL·min^-1. The detection wavelength was 328 nm, and the column temperature was set at 30 ℃. RESULTS 1,2,2'-tri-O-E-sinapoyl-β-gentiobiose and 1,2,6'-tri-O-E-sinapoylgentiobiose were showed good linear relationships within the ranges of 2.092-104.6 μg·mL^-1(R²=0.999 6) and 2.996-149.8 μg·mL^-1(R²=0.999 5). The average recovery (n=6) were 99.05% and 99.60%, RSDs were 0.34% and 0.65%, respectively. CONCLUSION The established method is simple, reliable and specific, and suitable for simultaneous determination of two components of phenolic acids glycosides in Isatidis Folium.