UHPLC-MS/MS同时测定白芷中34种植物生长调节剂残留量

Simultaneous Determination of 34 Plant Growth Regulator Residues in Angelicae Dahuricae Radix by UHPLC-MS/MS

关键词:  植物生长调节剂  白芷  残留量  超高效液相色谱-串联质谱法

Key words:  plant growth regulator  Angelicae Dahuricae Radix  residue  UHPLC-MS/MS

摘要:

目的 基于UHPLC-MS/MS建立同时测定白芷中34种植物生长调节剂残留量的分析方法。方法 样品通过乙腈高速匀浆提取，浓缩后直接进样，采用Waters Acquity UPLC HSS T3(2.1 mm×150 mm，1.8 µm)色谱柱，流动相0.1%甲酸水溶液含10 mmol·L^–1甲酸铵(A)-乙腈(B)，用基质匹配标液外标法进行定量。结果 34种植物生长调节剂在基质中的线性关系良好，相关系数均>0.995，检出限为0.01~9.26 µg·kg^-1，平均回收率为72.4%~100.6%，RSD均<15%。采用该法对35批白芷样品中的植物生长调节剂残留情况进行筛查，其中19批样品中检测出6种植物生长调节剂。结论 建立的植物生长调节剂多残留检测方法灵敏度高，准确性好，有针对性，且前处理简单快速，可用于筛查和检测白芷中植物生长调节剂多残留量。

Abstract:

OBJECTIVE To establish an analytical method for the simultaneous determination of 34 plant growth regulators residues in Angelicae Dahuricae Radix based on UHPLC-MS/MS. METHODS The samples were extracted by acetonitrile high-speed homogenization, concentrated, injected directly without purification method, separated by a Waters Acquity UPLC HSS T3(2.1 mm×150 mm, 1.8 µm) column with mobile phase of 0.1% formic acid aqueous solution containing 10 mmol·L^-1 ammonium formate(A)-acetonitrile(B). The matrix-matched external standard method was used for quantitative analysis. RESULTS The calibration curves of 34 plant growth regulators showed good linearity with correlation coefficients >0.995. The limits of detection were 0.01-9.26 µg·kg^-1, and average recoveries of 72.4%-100.6%, the RSD <15%. The plant growth regulators in 35 batches of Angelicae Dahuricae Radix were analyzed by this method. CONCLUSION The established method is sensitive, accurate, targeted, and simple with rapid pre-treatment, and can be used for screening and detection of plant growth regulator multi-residues in Angelicae Dahuricae Radix.