微流控芯片快速检测技术在铁皮石斛农药残留检测中的应用研究

Study on Application of Microfluidic Chip Rapid Detection in the Detection of Pesticide Residues in Dendrobium Officinale

关键词:  微流控芯片  铁皮石斛  快速检测  农药残留

Key words:  microfluidic chip  Dendrobium officinale  rapid detection  pesticide residues

摘要:

目的 探究微流控芯片快速检测技术在铁皮石斛农药残留检测应用的可行性。方法 采用有机溶剂提取样本，并用QuEChERS dispersive SPE净化，通过离心式微流控芯片高通量筛选不同厂家的农药残留检测用酶，考察阴性样品基质干扰的情况，采用不同水平加标的方式，考察微流控芯片农药残留检测方法的灵敏度、重复性、准确性，并和质谱法测定的样品结果进行比较。结果 快速检测方法显著差异率：0.25；灵敏度：99.6%；特异性：96.4%；假阴性率：0.39%；假阳性率：3.57%；准确度：98.81%。采用微流控芯片农药残留快速检测方法与质谱法2种方法检测40批次样品，测定结果表明，2种测定方法结果高度一致，检测结果符合率均为100%。结论 开发的微流控农药残留芯片快速检测技术可用于铁皮石斛农药残留的检测，可以满足基层非专业人员针对大批量农药残留的筛查需求。

Abstract:

OBJECTIVE To explore the feasibility of microfluidic chip in the rapid detection of pesticide residues in Dendrobium officinale. METHODS The sample was extracted with organic solvent, and purified with QuEChERS dispersive SPE, high throughput screening of enzymes for pesticide residue detection from different manufacturers by centrifugal microfluidic chips. The matrix interference was investigated by negative samples with different horizontal adding methods. The sensitivity, repeatability sand accuracy of the microfluidic chip were investigated, and sample results determined by mass spectrometry were compared with them. RESULTS The results revealed that the significant difference rate of rapid detection method was 0.25; the sensitivity was 99.6%; the specificity was 96.4%; the false negative rate was 0.39%; the false positive rate was 3.57%; the accuracy was 98.81%. Two methods of rapid detection of pesticide residues by microfluidic chip and mass spectrometry were used to detect 40 batches of samples. The results indicated that the two methods had a high consistency, compliance rate of testing results were 100%. CONCLUSION Microfluidic chip rapid detection technology can be used for detecting pesticide residues in Dendrobium officinale, which can meet the screening needs of the basic non-professional person to detect the quality of large quantities of pesticide residues.