| 引用本文: | 潘红烨,朱凤,俞佳玉,顾月婷,胡蔚同,何厚洪,姚建标,高建青,胡江宁.油菜花粉通过提高bFGF和VEGF水平改善小鼠雄激素源性脱发[J].中国现代应用药学,2023,40(16):2203-2209. |
| PAN Hongye,ZHU Feng,YU Jiayu,GU Yueting,HU Weitong,HE Houhong,YAO Jianbiao,GAO Jianqing,HU Jiangning.Rape Pollen Promote Androgenic Alopecia Mice by Increasing bFGF and VEGF Levels[J].Chin J Mod Appl Pharm(中国现代应用药学),2023,40(16):2203-2209. |
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| 油菜花粉通过提高bFGF和VEGF水平改善小鼠雄激素源性脱发 |
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潘红烨1,2,3, 朱凤1,2, 俞佳玉1,2, 顾月婷3, 胡蔚同3, 何厚洪1,2, 姚建标1,2, 高建青3, 胡江宁1,2
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1.浙江康恩贝制药股份有限公司, 杭州 310052;2.浙江省中药制药技术重点实验室, 杭州 310052;3.浙江大学药学院, 杭州 310058
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| 摘要: |
| 目的 探索油菜花粉对小鼠雄激素源性脱发的改善作用并研究其作用机制。方法 设立空白组、模型组、阳性药组及给药组，通过脱毛后涂抹0.2%睾酮诱导建立小鼠雄激素源性脱发模型，采用市售5%米诺地尔为阳性药物，0.4 g·mL-1油菜花粉油溶液为给药组，分别观察小鼠毛发生长速度。通过扫描电子显微镜(scanning electron microscope，SEM)、病理组织HE染色观察小鼠毛发质量及毛囊情况。使用免疫荧光染色、免疫印迹试验检测皮肤中VEGF、bFGF水平，ELISA检测血清性激素及活性氧水平。结果 与模型组相比，油菜花粉能显著促进小鼠毛发生长，且能够改善小鼠毛发毛鳞片的状态。机制探究试验表明油菜花粉不能通过调节小鼠激素水平或抗氧化应激起到促进毛发再生作用，但油菜花粉可以提高造模部位皮肤血管生成相关因子bFGF和VEGF的表达。结论 油菜花粉可以促进雄激素源性脱发小鼠毛发再生，其作用机制可能是通过提高bFGF和VEGF水平，促进毛囊周围血管再生从而达到促进毛发再生作用。 |
| 关键词: 油菜花粉 雄激素源性脱发 毛发再生 血管生成 |
| DOI：10.13748/j.cnki.issn1007-7693.20222939 |
| 分类号:R284.1 |
| 基金项目: |
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| Rape Pollen Promote Androgenic Alopecia Mice by Increasing bFGF and VEGF Levels |
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PAN Hongye1,2,3, ZHU Feng1,2, YU Jiayu1,2, GU Yueting3, HU Weitong3, HE Houhong1,2, YAO Jianbiao1,2, GAO Jianqing3, HU Jiangning1,2
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1.Zhejiang CONBA Pharmaceutical Co., Ltd., Hangzhou 310052, China;2.Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology, Hangzhou 310052, China;3.College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China
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| Abstract: |
| OBJECTIVE To explore the improvement effect of rape pollen on androgenic alopecia mice and its mechanism. METHODS The blank group, model group, positive drug group and administration group were set up, the androgenic alopecia mice model was induced by applying 0.2% testosterone after hair removal. The hair growth rate of mice were observed by using 5% minoxidil as positive drug and 0.4 g·mL-1 rape pollen oil solution as administration group. The hair quality and follicle condition of mice were observed by scanning electron microscope(SEM) and HE staining of skin tissue, respectively. The level of VEGF and bFGF in skin were detected by immunofluorescence staining and Western blotting, while the level of serum sex hormones and reactive oxygen species were detected by ELISA. RESULTS Rape pollen could significantly promote the hair growth in mice and improve the state of mice hair scales compared with model group. Mechanism exploration experiments showed that rape pollen could not promote hair regeneration of mice by regulating hormone levels or anti-oxidative stress. However, rape pollen could increase the expression of bFGF and VEGF related to skin angiogenesis at the modeling site. CONCLUSION Rape pollen can promote hair regeneration in androgenic alopecia mice. Its mechanism may be that it promotes perifollicular vascular regeneration by increasing bFGF and VEGF level. |
| Key words: rape pollen androgenetic alopecia hair regeneration angiogenesis |
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