基于抗氧化活性谱效关系的不同来源藏药材“加哇”的质量评价

Quality Assessment of Jiaw based on Antioxidant Activity Spectrum-effect Relationship

关键词:  加哇  聚类分析  主成分分析  抗氧化  阿魏酸  含量测定  谱效关系

Key words:  Jiawa, cluster  analysis, principal  component analysis, antioxidant, ferulic  acid, content  determination, spectrum-effect  relationship

摘要:

目的 通过谱效关系评价不同来源藏药材“加哇”的质量。方法 采用相似度评价、聚类分析、主成分分析等方法对17批“加哇”药材HPLC指纹图谱进行模式识别；采用灰色关联度分析法,研究了“加哇”指纹图谱中13个共有峰与“加哇”DPPH自由基和ABTS自由基清除能力的关系,并对其中关联性较强,响应较高的阿魏酸进行了含量测定。结果 17批“加哇”样品的指纹图谱、抗氧化活性及阿魏酸的含量差异巨大,质量参差不齐,通过模式识别分成了A、B两大类。产于墨竹工卡县的A类样品与其它样品相似度太低,化学成分的种类和含量均差异很大；B类样品为市场主流的“加哇”样品,但组内差异巨大,其中产于林芝的抗氧化活性最好,阿魏酸含量最高；拉萨及周边的西藏凹乳芹抗氧化活性较好,阿魏酸含量居中；昌都阿坝地区的“加哇”样品抗氧化活性最差,阿魏酸含量最低。结论 阿魏酸与“加哇”的抗氧化能力密切相关,可作为评价藏药“加哇”质量的重要指标。

Abstract:

ABSTRACT: OBJECTIVE To evaluate the quality of tibetan medicine "Jiawa" by the spectrum-effect relationship study. METHODS The HPLC fingerprints of 17 batches of "Jiawa" were analyzed by similarity evaluation, cluster analysis and principal component analysis. Gray correlation degree analysis was used to study the relationship between 13 common fingerprint peaks and DPPH / ABTS radical scavenging capacity of "Jiawa". Due to high correlation with antioxidant activity, the content of ferulic acid was determined. RESULTS 17 batches of "Jiawa" samples showed varied greatly in fingerprint characteristics, antioxidant activity and content of ferulic acid, which divided into group A and group B. Samples in group A from Mozhugongka, showed low similarity in both chemical components and concentration. Group B including 15 samples, which mainstreaming "Jiawa" products in the market, showed some difference within group. Samples from Linzhi showed the best antioxidant activity and the highest of ferulic acid; while those from Lhasa was in medium levels，and samples from Aba and Changdu has the minimum level in both antioxidant activity and ferulic acid content. CONCLUSION Ferulic acid is closely related to the antioxidant capacity of "Jiawa", which can be used as an important target to evaluate the quality of Tibetan medicine "Jiawa".