| 引用本文: | 王耀登,连艳,郭小红,蒋桂华,杨敏.HS-GC-IMS结合化学计量学快速鉴别不同产地枳壳[J].中国现代应用药学,2025,42(2):31-30. |
| wangyaodeng,lian yan,guo xiao hong,jiang gui hua,yang min.Keywords: Aurantium fructus;different origins; volatile components;HS-GC-IMS; chemometrics methods[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(2):31-30. |
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| 摘要: |
| 摘要：目的： 建立快速分析枳壳挥发性成分的方法，比较不同产地枳壳挥发性成分的差异。方法：采用顶空气相离子迁移谱技术（HS-GC-IMS）分析不同产地枳壳的挥发性成分，运用聚类分析（CA）、主成分分析法（PCA）和偏最小二乘法-判别分析（PLS-DA）进行不同产地枳壳分类。结果：共检测出80种挥发性化合物，鉴别其中55种，包含萜烯类13种、醛类18种、酯类5种、酮类8种、醇类8种、酸类2种、吡嗪类1种；通过聚类分析和主成分分析法可将不同产地枳壳进行区分。结论：该方法简便、经济、高效、直观，可用于识别和评价不同产地枳壳挥发性有机物的种类和差异，为枳壳产地鉴别和质量控制提供新技术。 |
| 关键词: 枳壳 不同产地 挥发性成分 顶空-气相色谱-离子迁移谱（HS-GC-IMS） 化学计量学 |
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| 基金项目:基金项目：国家自然科学基金项目（No.82173928），四川省自然科学基金（No.2022NSFSC1548），重庆英才计划创新领军人才项目（No.cstc2022ycjh-bgzxm0207），成都中医药大学“杏林学者”学科人才科研提升计划（No.YYZX2022139） |
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| Keywords: Aurantium fructus;different origins; volatile components;HS-GC-IMS; chemometrics methods |
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wangyaodeng1, lian yan1, guo xiao hong2, jiang gui hua1, yang min1
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1.Chengdu University of Traditional Chinese Medicine;2.Chongqing Traditional Chinese Medicine Hospital
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| Abstract: |
| ABSTRACT: OBJECTIVE To distinguish the different origins of Aurantium fructus by headspace-gas-ion mobility spectroscopy (HS-GC-IMS) combined with chemometrics. METHODSThe analysis was performed on an MXT-WAX column (30 m×0.53 mm, 1 μm); column temperature 60°C; separation time 30 min; migration tube temperature 45; migration gas volume flow rate 150 mL/min. The volatile components of Aurantium fructus of different origins were detected, and the qualitative analysis was performed by NIST and IMS databases, and the plot differences were compared by Reporter and Gallery Plot plug-ins, combined with cluster analysis (CA), principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) to compare the differences and similarities of volatile components of Aurantium fructus of different origins.RESULTSA total of 80 volatile compounds were detected, and 55 were identified qualitatively, including 13 terpenes, 18 aldehydes, 5 esters, 8 ketones, 8 alcohols, 2 acids and 1 pyrazine; the different origins of Aurantium fructus can be distinguished by cluster analysis (CA) and principal component analysis (PCA). COMCLUSIONThe method is simple, environmentally friendly and efficient, and can be used to identify and evaluate the types and differences of volatile organic compounds in Aurantium fructus shells of different origins, providing a new technique for the identification and quality control of Aurantium fructus of different origins. |
| Key words: Aurantium fructus different origins volatile components HS-GC-IMS chemometrics methods |
