艾绒中酸性黄36快速检测方法研究

Study on rapid detection methods of acid yellow 36 in moxibustion

关键词:  艾绒  酸性黄36  快检方法  HPLC-DAD法  LC-MS法

Key words:  moxibustion  acid yellow 36  rapid detection methods  HPLC-DAD  LC-MS/MS

摘要:

目的：建立艾绒中非法添加色素酸性黄36的快速检测方法。方法：利用酸性黄36为酸碱指示剂,在pH值1.2(红)～2.3(黄)范围内变色的原理,以15%硫酸溶液为显色剂，对样品乙醇提取液进行初筛，再采用HPLC法对初筛可疑阳性样品进行验证，最后采用LC-MS法进行确认,检验所建立的理化快检方法的准确性。结果：应用建立的酸性黄36快检方法在67批艾绒样品中检出3批阳性样，经HPLC法和LC-MS法确认后，结果一致。结论：本文所建立的酸性黄36快速测定方法准确、快速、便捷,可开发为现场快检方法,为监管部门现场有效打击艾绒中非法添加酸性黄36的行为提供有力的技术支撑。

Abstract:

ABSTRACT: OBJECTIVE To establish a rapid method for determining of acid yellow 36 in moxibustion. METHODS Based on the principle of acid yellow 36 as an acid-base indicator and discoloration in the pH range of 1.2 (red) to 2.3 (yellow), 15% sulfuric acid solution was used as the color developing agent to screen the ethanol extract of the sample, and then HPLC method was used to verify the suspicious positive samples in the initial screening, and finally LC-MS method was used to confirm the accuracy of the established rapid physicochemical detection method. RESULTS The established method was applied to 67 batches of samples, and three positive samples were detected. The results were consistent with those of HPLC and LC-MS. CONCLUSION The method is accurate and sensitive ,which can be developed as a fast detection method on the spot, and provide strong technical support for the regulatory authorities to crack down on the illegal addition of acid yellow 36 in moxibustion.