| 引用本文: | 陶培,刘丛改,红花,拉巴次仁,陈佳佳,郑清洋,泽翁拥忠,王毓杰.短柄乌头中二萜生物碱类成分及心脏毒性研究[J].中国现代应用药学,2024,41(18):106-113. |
| TAO Pei,LIU Conggai,Hong Hua,LABA Ciren,CHEN Jiajia,ZHENG Qingyang,ZEWENG Yongzhong,WANG Yujie.Diterpenoid Alkaloids from Aconitum brachypodum and Their Cardiotoxicity[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(18):106-113. |
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| 摘要: |
| 目的 研究短柄乌头块根中二萜生物碱的结构及心脏毒性。方法 采用硅胶柱色谱及波谱法分离鉴定化合物的结构；采用大鼠模型比较不同化合物的心脏毒性。结果 从短柄乌头中分离得到12个二萜生物碱，分别鉴定为：secokaraconitine(1)、oxonitine(2)、查斯曼宁(3)、草乌甲素(4)、尼奥林(5)、去氧乌头碱(6)、乌头碱(7)、印乌碱(8)、滇乌碱(9)、塔拉萨敏(10)、苯甲酰乌头原碱(11)、乌头原碱(12)。通过整体动物心脏毒性实验证实：1-OH、3-OH、8-COCH3、14-苯甲酰基能够增加乌头烷类化合物的心脏毒性，6-OCH3、N-CHO、N=C能够降低该类化合物的心脏毒性。结论 通过整体动物实验明确了不同取代基对乌头烷类化合物心脏毒性的影响。 |
| 关键词: 乌头属 短柄乌头 二萜生物碱 心脏毒性 secokaraconitine oxonitine |
| DOI： |
| 分类号:R284.1；R917.101 |
| 基金项目:四川省科技计划项目（2020YJ0131）；成都中医药大学“杏林学者”学科人才科研提升计划（QJRC2022046） |
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| Diterpenoid Alkaloids from Aconitum brachypodum and Their Cardiotoxicity |
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TAO Pei1, LIU Conggai1, Hong Hua2, LABA Ciren3,4,5, CHEN Jiajia1, ZHENG Qingyang1, ZEWENG Yongzhong1, WANG Yujie1
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1.Chengdu University of Traditional Chinese Medicine;2.Nyingchi Hospital of Tibetan Medicine;3.Nyingchi Gongbo'4.'5.gyamda County Jiangda Township Health Center
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| Abstract: |
| OBJECTIVE To study the diterpenoid alkaloids from the roots of Aconitum brachypodum and to compare the cardiotoxicity of these compounds. METHODS The chemical constituents were isolated and purified using silica gel column chromatography. Their structures were established on the basis of their spectroscopic data and comparison with those of the literature. Furthermore, cardiotoxicity assay was used to compare the toxicity of these compounds. RESULTS Twelve diterpenoid alkaloids were isolated and identified as secokaraconitine(1), oxonitine(2), chasmanine(3), crassicauline A(4), neoline(5), deoxyaconitine(6), aconitine(7), indaconitine(8), yunaconitine(9), talatisamine(10), benzoylaconine(11), and aconine(12). Through cardiotoxicity assay, it has been confirmed that 1-OH, 3-OH, 8-COCH3, and 14-benzoyl group can increase cardiotoxicity, while 6-OCH3, N-CHO and N=C can reduce it. CONCLUSION The effects of different substituents on the cardiotoxicity of aconitane compounds were clarified through overall animal experiments. |
| Key words: Aconitum L. Aconitum brachypodum diterpenoid alkaloid cardiotoxicity secokaraconitine oxonitine |
