覆盆子指纹图谱及多成分含量测定方法研究

Fingerprint analysis and multi-component determination of Rubus chingii HuCHEN Xinxin, SHI Xiaowei, MA Peiyuan, CHEN Shantan, ZHENG Hongyue, LI Fanzhu* (Zhejiang Chinese Medical University, Hangzhou 310053, China)

关键词:  覆盆子  指纹图谱  多成分含量测定  品质评价

Key words:  Rubus chingii Hu  fingerprint  multi-component determination  quality evaluation

摘要:

目的 建立覆盆子指纹图谱,结合多指标定量分析方法测定有效成分对不同产区的覆盆子进行品质评价。方法 运用紫外分光光度法、高效液相色谱(HPLC)法、电位滴定法测定10批不同产区覆盆子中总黄酮、山奈酚、山奈酚-3-O-芸糖苷、银锻苷、覆盆子苷-F5、鞣花酸和总有机酸含量并建立相关指纹图谱,对其品质进行评价。结果 10批不同产区覆盆子成分差异较大,经紫外分光光度法测定得到浙江淳安产区总黄酮(以芦丁计)含量最高。10批覆盆子指纹图谱共标记有20个共有峰,其中安徽巢湖产区相似度最低仅为0.576,最高的为浙江金华产区相似度为0.913。HPLC多指标含量测定得到山奈酚含量以湖北蕲春产区最高,山奈酚-3-O-芸糖苷和银锻苷含量以浙江新昌产区最高,覆盆子苷-F5含量以江西上饶产区最高,鞣花酸含量以浙江淳安产区最高。此外,通过电位滴定法测定覆盆子中总有机酸(以琥珀酸计),四川德阳、浙江淳安、江苏昆山三个产区含量最高,分别为1.58%、1.59%、1.60%。结论 本文建立的覆盆子多成分含量测定方法稳定、可靠、高效,为其品质评价提供了有效参考。

Abstract:

OBJECTIVE To evaluate the quality of Rubi Fructus from different production areas, multi-index quantitative analysis methods was established. METHODS The flavonoids, kaempferol, kaempferol-3-O-rutinose, tilirosid, goshonoside-F5, ellagic acid and total organic acids in 10 batches of Rubi Fructus from different regions were determined using ultraviolet spectrophotometry, HPLC and potentiometric titration. And the quality assessment of Rubi Fructus was carried out based on HPLC fingerprints. RESULTS There were significant differences in the components of the 10 batches of Rubi Fructus from different regions. The total flavonoids content (calculated as rutin) in Rubi Fructus (Chun'an, Zhejiang Province) exhibited the highest among those from the Chun'an region of Zhejiang Province, as determined by ultraviolet spectrophotometry. The fingerprints of 10 batches of raspberries marked 20 common peaks, among which the lowest similarity was 0.576 in that of Chaohu, Anhui production area, and the highest was in that of Jinhua, Zhejiang production area, which was close to Zhejiang Chun'an. The results of the multi-index content determination by HPLC showed that the highest content regions of kaempferol, kaempferol-3-O-glycoside, silverthornin, raspberry glycoside-F5, and ellagic acid were Qichun, Hubei production area, Xinchang, Zhejiang, Xinchang, Zhejiang, Shangrao, Jiangxi, and Chun'an, Zhejiang production area, respectively. In addition, the total organic acids in raspberry (calculated as succinic acid) were determined by potentiometric titration, with the highest contents found in Deyang, Sichuan Province, Chun'an, Zhejiang Province and Kunshan, Jiangsu Province, respectivel. CONCLUSION The established quantitative analysis method is stable, reliable, and efficient, which provides effective reference for the quality evaluation of Rubi Fructus.