共载榄香烯和纳米氧化石墨烯的可注射型脂质体水凝胶用于4T1乳腺癌治疗研究

王子昂; 李一菁; 陈家琦; 朱艳慧; 王白燕; 贾永艳; 祝侠丽

引用本文:	王子昂,李一菁,陈家琦,朱艳慧,王白燕,贾永艳,祝侠丽.共载榄香烯和纳米氧化石墨烯的可注射型脂质体水凝胶用于4T1乳腺癌治疗研究[J].中国现代应用药学,2024,41(19):70-78.
	WANG Ziang,LI Yijing,CHEN Jiaqi,ZHU Yanhui,WANG Baiyan,JIA Yongyan,ZHU Xiali.Injectable liposome hydrogel co-loading elemene and nano graphene oxide for therapy of 4T1 breast cancer[J].Chin J Mod Appl Pharm(中国现代应用药学),2024,41(19):70-78.

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共载榄香烯和纳米氧化石墨烯的可注射型脂质体水凝胶用于4T1乳腺癌治疗研究
王子昂¹, 李一菁¹, 陈家琦¹, 朱艳慧¹, 王白燕², 贾永艳¹, 祝侠丽¹
1.河南中医药大学药学院;2.河南中医药大学基础医学院

摘要:

目的结合808 nm激光照射,探讨共载榄香烯(ELE)和甘草次酸修饰的纳米氧化石墨烯(GA/NGO)的可注射型脂质体水凝胶(Lip-gel)用于4T1乳腺癌光热-化协同靶向治疗。方法以4T1乳腺癌细胞为模型,采用MTT、激光共聚焦等技术进行细胞抑制和摄取试验；以4T1雌性BALB/c小鼠为模型,采用红外热成像、活体成像、ELISA等技术评价体内分布和抗肿瘤活性。结果与ELE溶液相比,ELE-GA/NGO-Lip-gel能够显著抑制4T1细胞的增殖作用(P<0.001),并且具有浓度和时间依赖性。经瘤内注射给药后,ELE-GA/NGO-Lip-gel在荷瘤小鼠体内具有良好的光热转换效应,在肿瘤组织的滞留时间较长。药效学试验中ELE-GA/NGO-Lip-gel组小鼠体重无明显变化、相对肿瘤体积变化较小,H E和TUNEL病理切片结果显示ELE-GA/NGO-Lip-gel可使肿瘤细胞大面积凋亡。ELE-GA/NGO-Lip-gel可显著降低小鼠血清中TNF-α、IFN-γ、IL-6的含量(P<0.001)。结论 ELE-GA/NGO-Lip-gel结合近红外光照射对4T1乳腺癌的体内外抗肿瘤活性增强。

关键词: 榄香烯纳米氧化石墨烯脂质体水凝胶光热-化治疗 4T1乳腺癌

DOI：

分类号:

基金项目:国家自然科学基金资助项目（No.81803740）；河南省高等学校重点科研项目（No. 23A360007）

Injectable liposome hydrogel co-loading elemene and nano graphene oxide for therapy of 4T1 breast cancer

WANG Ziang¹, LI Yijing¹, CHEN Jiaqi¹, ZHU Yanhui¹, WANG Baiyan², JIA Yongyan¹, ZHU Xiali¹

1.Pharmacy College，Henan University of Chinese Medicine;2.Basic Medicine College，Henan University of Chinese Medicine

Abstract:

OBJECTIVE Combined with 808 nm laser irradiation, the injectable liposome hydrogel (ELE-GA/NGO-Lip-gel) loaded with elemene (ELE) and glycyrrhetinic acid modified nano graphene oxide (GA/NGO) was investigated for photothermal and synergistic targeted therapy of 4T1 breast cancer. METHODS 4T1 breast cancer cell was taken as a model, MTT and laser confocal technology were used for the antitumor activity and uptake test. 4T1 bearing female BALB/c mice was taken as a model, infrared thermal imaging, in vivo imaging, ELISA and other techniques were used to evaluate the biodistribution and pharmacodynamics of ELE-GA/NGO-Lip-gel. RESULTS Compared with ELE solution, ELE-GA/NGO-Lip-gel significantly inhibited the proliferation of 4T1 cells (P<0.001) in a concentration - and time-dependent manner. After intratumoral injection, ELE-GA/NGO-Lip-gel had a good photothermal conversion effect in tumor-bearing mice and a longer retention time in tumor tissues. In the pharmacodynamic test, ELE-GA/NGO-Lip-gel group showed no significant changes in body weight and relative tumor volume, and the results also showed that ELE-GA/NGO-Lip-gel could induce large area apoptosis of tumor cells, and significantly reduced the levels of TNF-α, IFN-γ and IL-6 in serum of mice (P<0.001). CONCLUSION ELE-GA/NGO-Lip-gel combined with near infrared light irradiation could enhance the antitumor activity of 4T1 breast cancer in vivo and in vitro.

Key words: elemene, nano graphene oxide liposome hydrogel chemo-photothermal therapy 4T1 breast cancer