新型活血贴膏与原散剂治疗慢性软组织损伤的药效学比较研究

Pharmacodynamic Comparative of the Novel Huoxue Plaster and the Original Powder in Treating Chronic Soft Tissue Injury

关键词:  活血贴膏  活血散  抗炎  镇痛  慢性软组织损伤

Key words:  Huoxue plaster  Huoxue powder  anti-inflammatory effect  analgesia  soft tissue injury

摘要:

目的 比较新型活血贴膏与原散剂在治疗慢性软组织损伤方面的疗效差异，为医院制剂研发及临床应用提供实验依据。方法 按照给药不同，分别设立空白组、基质组、阳性对照组(扶他林组)、原散剂生品组、原散剂制品组、新型贴膏剂组，通过小鼠扭体试验、甲醛致痛试验、电刺激试验和热板试验测定扭体抑制率、舔足时间、镇痛率及痛阈值比较镇痛作用的药效差异；通过小鼠耳肿胀试验和毛细血管通透性试验测定肿胀度、肿胀抑制率和渗出抑制率比较抗炎作用的药效差异；建立大鼠慢性软组织损伤模型，通过大鼠小腿周长变化、软组织损伤外观评分、血液流变学组织病理形态学观察和炎性因子含量变化比较处方及剂型改革前后的药效差异。结果 新型活血贴膏对醋酸和甲醛导致的小鼠炎性疼痛具有显著的抑制效果，与原散剂相比无明显差异；对电刺激引起的小鼠疼痛有明显镇痛作用，其中给药后60 min的镇痛效果优于给药后90 min和120 min的效果，与原散剂相比无明显差异；新型活血贴膏与原散剂对热板痛阈值均无明显作用。新型活血贴膏具有显著的抗炎作用，可以减轻二甲苯致小鼠耳肿胀程度，减少醋酸致毛细血管的炎性渗出，且与原散剂相比差异无统计学意义。新型活血贴膏能显著减少慢性软组织损伤模型大鼠肿胀小腿的周长和损伤症候指数，改善血液循环，改善组织形态学，显著减少损伤软组织中PGE2、TNF-α、IL-1β的含量，且与原散剂相比差异无统计学意义。结论 新型活血贴膏具有显著的镇痛、抗炎作用，可用于治疗慢性软组织损伤，药物疗效与原散剂基本一致。

Abstract:

OBJECTIVE To compare the efficacy of the novel Huoxue plaster and the original powder in the treatment of chronic soft tissue injury, and to provide experimental basis for the development and clinical application of hospital preparations. METHODS According to the different drug administration, mice were assigned to the blank group, the matrix group, the positive control group(voltaren group), the original powder raw product group, the original powder product group and the novel plaster group respectively. The writhing inhibition rate, licking time, analgesic rate and pain threshold were measured by the writhing test, formaldehyde pain test, electrical stimulation test and hot plate test. The purpose of these was to assess disparities in the drugs for analgesic purposes. The mice ear swelling test and capillary permeability test were used to determine the swelling degree, swelling inhibition rate and exudation inhibition rate to compare the differences in anti-inflammatory effects. A rat model of chronic soft tissue injury was established. The changes of calf circumference, soft tissue injury appearance score, hemorheology and histopathological observation and inflammatory factor content were compared before and after the reform of prescription and dosage form. RESULTS The novel Huoxue plaster had a significant inhibitory effect on the inflammatory pain induced by acetic acid and formaldehyde in mice, and there was no significant difference compared with the original powder. The analgesic effect of 60 min after administration was better than that of 90 min and 120 min after administration, and there was no significant difference compared with the original powder. The novel Huoxue plaster and the original powder had no significant effect on hot plate pain threshold. The novel Huoxue plaster had a significant anti-inflammatory effect, which could reduce the degree of ear swelling induced by xylene and reduce the inflammatory exudation of capillaries induced by acetic acid, and there was no significant difference compared with the original powder. The novel Huoxue plaster could significantly reduce the circumference and injury symptom index of the swollen leg in rats with chronic soft tissue injury, improve blood circulation and histomorphology, and significantly reduce the contents of PGE2, TNF-α and IL-1β in the injured soft tissue, and there was no significant difference compared with the original powder. CONCLUSION The novel Huoxue plaster has significant analgesic and anti-inflammatory effects, and can be used to treat chronic soft tissue injury. The efficacy of the novel Huoxue plaster is basically the same as that of the original powder.