UHPLC-MS/MS测定不同产地市售南五味子药材中8种木脂素成分

Determination of Eight Lignans in Schisandrae Sphenantherae Fructus on the Market from Different Habitats by UPLC-MS/MS

关键词:  南五味子  木脂素  安五脂素  北五味子  超高效液相色谱-串联质谱

Key words:  Schisandrae Sphenantherae Fructus  lignan  anwuligan  Schisandra chinensis (Turcz.) Baill. Fruits  UPLC-MS/MS

摘要:

目的 比较12个产地市售南五味子中五味子甲素、五味子乙素、五味子丙素、五味子醇甲、五味子醇乙、五味子酯甲、安五脂素、五味子酚的含量差异。方法 样品用96孔蛋白沉淀微孔板进行前处理，采用ACE EXCEL 1.7 C₁₈-AMIDE(100 mm×2.1 mm，1.7 μm)色谱柱，以0.1%甲酸水溶液(A)和甲醇(B)作为流动相进行梯度洗脱，流速为0.4 mL·min^?1。采用电喷雾离子源，正离子模式，多离子反应监测模式扫描。考察该方法的线性、仪器精密度、稳定性、重复性、加样回收率。结果 12个产地中10个产地的五味子酯甲含量均达到中国药典2020年版对南五味子规定的≥0.2%的标准。结论 UHPLC-MS/MS适宜南五味子中多成分的同时定量检测；市场所售南五味子品种基本符合国家法定标准。

Abstract:

OBJECTIVE To compare the contents of schizandrin A, schizandrin B, schizandrin C, schisandrol A, schisandrol B, schisantherin A, anwuligan, and schisanhenol in Schisandrae Sphenantherae Fructus on market from 12 habitats. METHODS The samples were pre-treated by 96-well fitration plates. The assay was performed on ACE EXCEL 1.7 C₁₈-AMIDE(100 mm×2.1 mm, 1.7 μm) column with 0.1% formic acid-water(A) and methanol(B), gradient elution, flow speed was 0.4 mL·min^–1. Ion source was electric spray ion source, positive ion monitoring mode, multireaction monitoring mode for quantitative analysis. Linear, instrument precision, stability, repeatability, average recovery were investigated. RESULTS The content of schisantherin A in 10 of 12 producing areas reached the standard of ≥0.2% of Schisandrae Sphenantherae Fructus in 2020 Edition of Chinese Pharmacopoeia. CONCLUSION UHPLC-MS/MS is suitable for simultaneous determination of multiple components in Schisandrae Sphenantherae Fructus. The Schisandrae Sphenantherae Fructus in the market basically meet the national legal standards.