水蛭联合全蝎冻干粉对大鼠颈动脉血栓形成及凝血功能和纤溶系统的影响

Effect of Leech Combined with Whole Scorpion Lyophilized Powder on Carotid Artery Thrombosis, Coagulation Function and Fibrinolysis System in Rats

关键词:  水蛭  全蝎  冻干粉  血栓形成  凝血功能  纤溶系统

Key words:  leech  scorpion  freeze-dried powder  thrombosis  coagulation function  fibrinolytic system

摘要:

目的 探讨水蛭联合全蝎冻干粉对大鼠颈动脉血栓形成的影响。方法 采用三氯化铁诱导颈动脉血栓形成，实验分6组，即假手术组、模型组、水蛭冻干粉组(水蛭组)、全蝎冻干粉组(全蝎组)、水蛭+全蝎半剂量组(半剂量组)、水蛭+全蝎全剂量组(全剂量组)，每组6只大鼠。末次给药后计算两侧颈总动脉血栓形成抑制率。采用血小板聚集及凝血因子分析仪测定活化部分凝血活酶时间(activated partial thromboplastin time，APTT)、血浆凝血酶原时间(prothrombin time，PT)、血浆纤维蛋白原(fibrinogen，FIB)和凝血酶原时间(thrombin time，TT)。采用ELSIA检测血浆组织纤溶酶原激活物(tissue-type plasminogen activator，t-PA)、I型纤溶酶原激活物抑制因子(plasminogen activator inhibitor-I，PAI-I)。HE染色观察各组血栓形成情况。结果 与模型组相比，水蛭组、全蝎组、半剂量组和全剂量组中血栓湿重和干重均降低，全剂量组抑制血栓形成效果最佳。与假手术组相比，模型组APTT、PT、TT缩短，FIB含量增加，t-PA水平下降；与模型组相比，各给药组中APTT、PT、TT时间延长，FIB含量减少，t-PA水平增加。PAI-I在各组中差异无统计学意义。模型组血管内均可见大量血栓。与模型组相比，水蛭组、全蝎组和半剂量组血栓明显减少，全剂量组几乎没有明显血栓。结论 水蛭联合全蝎冻干粉通过影响凝血功能和纤溶系统抑制大鼠颈动脉血栓形成。

Abstract:

OBJECTIVE To investigate the effect of leech freeze-dried powder combined with scorpion freeze-dried powder on carotid artery thrombosis in rats. METHODS FeCl₃ was used to induce carotid artery thrombosis, and the experiment was divided into 6 groups: sham-operated group(sham group), model group, leech freeze-dried powder group(leech group), whole scorpion freeze-dried powder group(scorpion group), leech+scorpion half-dose group(half-dose group), leech+scorpion full-dose group(full-dose group), 6 rats in each group. The inhibition rate of bilateral carotid artery thrombosis was calculated after the final administration. The activated partial thromboplastin time(APTT), plasma prothrombintime(PT), plasma fibrinogen(FIB), and prothrombin time(TT) were measured by platelet aggregation and coagulation factor analyzer. The levels of plasma tissue-type plasminogen activator(t-PA) and plasminogen activatorinhibitor-I(PAI-I) were measured by ELSIA, and the thrombosis of each group was observed by HE staining. RESULTS Compared with model group, the wet weight and dry weight of thrombus were reduced in the leech, scorpion, half-dose and full-dose groups, and the full-dose group had the best effect in inhibiting thrombus formation. Compared with sham group, APTT, PT, TT was shortened, FIB content increased and t-PA level decreased in model group; compared with model group, APTT, PT, TT was prolonged, FIB content decreased and t-PA level increased in each dose group. The PAI-Ⅰ was not significantly different in each group. A large number of thrombus were seen in the blood vessels of model group. Compared with model group, there were significantly fewer thrombus in the leech, scorpion and half-dose groups, and almost no significant thrombus in the full-dose group. CONCLUSION Leech freeze-dried powder combined with scorpion freeze-dried powder inhibited carotid artery thrombosis in rats by affecting coagulation function and fibrinolytic system.