达立通颗粒挥发油GC指纹图谱及GC-MS成分分析

GC Fingerprint and GC-MS Composition Analysis of Volatile Oil in Dalitong Granules

关键词:  达立通颗粒  挥发油  指纹图谱  GC-MS

Key words:  Dalitong granules  volatile oil  fingerprint  GC-MS

摘要:

目的 建立达立通颗粒挥发油GC指纹图谱，并对挥发油成分进行分析。方法 以Agilent HP-5(30 m×0.32 mm，0.25 μm)石英毛细管柱进行分离，程序升温，氢火焰离子化检测器检测。建立10批样品图谱共有模式，结合化学计量学法分析。运用GC-MS与NIST11.L标准谱库检索，鉴定挥发油成分，并推测各成分来源。结果 从10批样品共有图谱中选取13个共有峰，各成分相似度均>0.998，样品可分为3类。通过GC-MS共鉴定出56种成分，包含11个共有峰。结论 建立的达立通颗粒挥发油GC指纹图谱仪器精密度、重复性与稳定性良好，可为达立通颗粒挥发油质量控制提供参考依据。

Abstract:

OBJECTIVE To establish the GC fingerprint of the volatile oil of Dalitong granules, and analyze the volatile oil components. METHODS The separation was performed on an Agilent HP-5(30 m×0.32 mm, 0.25 μm) quartz capillary column with programmed temperature and hydrogen flame ionization detector. A common pattern of 10 batches of samples was established and analyzed by a combination of chemometric methods. The volatile oil components were identified using GC-MS with NIST11.L standard library, and the origin of each component was inferred. RESULTS Thirteen common peaks were selected from the common chromatograms of 10 batches of samples, and the similarity of each component was >0.998, and the samples could be classified into three categories. A total of 56 components were identified by GC-MS, including 11 common peaks. CONCLUSION The GC fingerprint of Dalitong granules volatile oil was established with good instrument precision, reproducibility and stability, which can provide a reference basis for the quality control of Dalitong granules volatile oil.