基于化学计量学的浙产醋延胡索镇痛的物质基础研究

Study on the analgesic material basis of Zhejiang vinegar Corydalis Rhizoma based on chemometrics

关键词:  浙产醋延胡索  指纹图谱  镇痛  化学计量学  物质基础

Key words:  zhejiang  vinegar corydalis rhizoma  fingerprint  analgesia  chemometrics  material basis

摘要:

目的 探讨浙产醋延胡索HPLC指纹图谱与镇痛药效的相关性，筛选出浙产醋延胡索发挥镇痛作用的物质基础。方法 建立浙产醋延胡索HPLC指纹图谱与大鼠痛经模型，采用大鼠体重下降率、扭体反应、血清丙二醛（MDA）、雌二醇（E2）、前列腺素E2（PGE2）及前列腺素F2α（PGF2α）的水平作为镇痛药效评价指标，结合化学计量学筛选浙产醋延胡索发挥镇痛作用的主要活性成分以及对含量贡献度较大的重要性成分。结果 建立了不同批次浙产醋延胡索饮片的指纹图谱，指认了9个成分；相比空白组，模型组的体重下降率、扭体反应、MDA、E2、PGF2α水平均显著升高，相比模型组，给药组均有所降低，相比空白组，模型组的PGE2水平显著降低，相比模型组，给药组的PGE2水平显著升高；浙产醋延胡索具有较好的镇痛效果，其发挥镇痛作用的主要活性成分为原阿片碱、盐酸巴马汀、脱氢紫堇碱；对含量贡献度较大的重要性成分为延胡索乙素、原阿片碱、盐酸巴马汀与四氢黄连碱。结论 浙产醋延胡索的药效发挥是多成分共同作用的结果，各成分与药效学指标具有较强的相关性，本研究为浙产醋延胡索镇痛的物质基础筛选提供了参考依据。

Abstract:

Objectives To explore the correlation between HPLC fingerprint and analgesic efficacy of Zhejiang vinegar Corydalis Rhizoma, and to screen out the material basis of analgesic effect of Zhejiang vinegar Corydalis Rhizoma. Methods To establish HPLC fingerprint of Zhejiang vinegar Corydalis Rhizoma and dysmenorrhea model in rats. The weight loss rate, writhing response, serum malondialdehyde (MDA), estradiol (E2), prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) levels were used as the evaluation indexes of analgesic efficacy. Combined with chemometrics, the main active components of analgesic effect of Zhejiang vinegar Corydalis Rhizoma and the important components with great contribution to the content were screened. Results The fingerprints of different batches of Zhejiang vinegar Corydalis Rhizoma were established, and nine components were identified; compared with the blank group, the weight loss rate, writhing reaction, MDA, E2 and PGF2α levels in the model group were significantly increased. Compared with the model group, the administration group was decreased. Compared with the blank group, the PGE2 level in the model group was significantly decreased. Compared with the model group, the PGE2 level in the administration group was significantly increased. The vinegar Corydalis Rhizoma produced in Zhejiang has a good analgesic effect, and the main active components of its analgesic effect are protopine, palmatine hydrochloride, and dehydrocorydaline. The important components with greater contribution to the content were tetrahydropalmatine, protopine, palmatine hydrochloride and stylopine. Conclusion The efficacy of Zhejiang vinegar Corydalis Rhizoma was the result of the combined action of multiple components, and each component had a strong correlation with the pharmacodynamic indexes. This study provided a reference for the screening of analgesic material basis of Zhejiang vinegar Corydalis Rhizoma.