金水六君煎对哮喘小鼠肺水代谢及TNF-α/NF-κB信号通路的影响

Effect of Jinshui Liujun Decoction on Pulmonary Water Metabolism and TNF-α/NF-κB Signaling Pathway in Asthmatic Mice

关键词:  金水六君煎  哮喘  肺水代谢  水通道蛋白  TNF-α/NF-κB信号通路

Key words:  Jinshui Liujun decoctions  asthma  pulmonary water metabolism  aquaporins  TNF-α/NF-κB signal pathway

摘要:

目的 研究金水六君煎对哮喘小鼠肺水代谢及TNF-α/NF-κB信号通路的影响。方法 将小鼠适应性饲养1周后，按体质量随机取10只作为空白对照组，其余小鼠实验第1 天、第8 天及第15天腹腔注射与皮下注射卵蛋白致敏液致敏，第22 天随机分为模型对照组、阳性对照组及金水六君煎低、中、高剂量(4.1，8.2，16.4 g·kg^-1)组，隔日1次、连续2周吸入雾化激发液(1% 卵蛋白)复制哮喘小鼠模型，同时每天灌胃给予受试药，观测金水六君煎对哮喘小鼠行为学、肺湿干重比值、AQP1、AQP5、TNF-α、NF-κB及NF-κB mRNA表达的影响。结果 金水六君煎可减少哮喘发作动物数，缓解哮喘症状，降低肺指数及肺湿干重比值，提高肺组织AQP1、AQP5的表达水平，降低支气管肺泡灌洗液及肺组织TNF-α的含量，下调肺组织NF-κB及其基因表达水平(P<0.05或P<0.01)。结论 金水六君煎具有一定的平喘作用，抑制TNF-α/NF-κB信号通路、上调AQP1和AQP5而改善肺水代谢是其作用机制之一。

Abstract:

OBJECTIVE To study effects of Jinshui Liujun decoction(JLD) on pulmonary water metabolism and TNF-α/NF-κB signaling pathway in asthmatic mice. METHODS After 1 week of acclimatization, 10 mice were randomly selected by body weight as a blank control group, and the rest of the mice were sensitized by intraperitoneal and subcutaneous injections of ovalbumin sensitizing solution on the 1st, 8th, and 15th day of the experiment. On the 22nd day, they were randomly divided into model control group, positive control group and JLD low, medium and high dose(4.1, 8.2, 16.4 g·kg^-1) group, and replicated the asthma mouse model by inhaling nebulized excitation solution(1% ovalbumin) once every other day for 2 consecutive weeks, and at the same time, were given atomized excitation solution(1% ovalbumin) every other day for two weeks to replicate the asthmatic mouse model, and were given the test drug by gavage daily. The effects of JLD on the behavior, lung wet-dry weight ratio, AQP1, AQP5, TNF-α, NF-κB, and NF-κB mRNA expression in asthmatic mice were observed. RESULTS JLD reduced the number of animals with asthma attacks, alleviated asthma symptoms, decreased the lung index and lung wet-to-dry weight ratio, increased the expression level of AQP1 and AQP5 in lung tissues, reduced the content of TNF-α in bronchoalveolar lavage fluid and lung tissues, and down-regulated the expression level of NF-κB and its genes in lung tissues(P<0.05 or P<0.01). CONCLUSION JLD has an anti-asthma effect and its mechanism of action is partially due to its inhibition of the TNF-α/NF-κB signaling pathway, upregulation of AQP1 and AQP5, and improvement of pulmonary water metabolism.