载隐丹参酮普鲁士蓝纳米粒的制备及评价

Preparation and evaluation of Cryptotanshinone-containing Prussian Blue Nanoparticles

关键词:  普鲁士蓝纳米粒子  隐丹参酮  光热治疗  抗肿瘤

Key words:  prussian blue nanoparticles  cryptotanshinone  photothermal therapy  anti-tumor

摘要:

目的：制备负载隐丹参酮的普鲁士蓝纳米粒 (Cryptotanshinone-containing Prussian Blue Nanoparticles，CPT@PB NPs)，并对制备得到的纳米粒的理化性质、光热性能及细胞毒性进行评价。方法：采用水热合成法和溶剂吸附法制备CPT@PB NPs；采用透射电子显微镜 (Transmission Electron Microscopy，TEM) 和动态光散射技术 (Dynamic Light Scattering，DLS) 对纳米粒进行表征；采用红外光热成像仪检测纳米粒的光热效应；最后，通过CCK-8法评估CPT@PB NPs的体外抗肿瘤活性。结果：制备的CPT@PB NPs呈规则立方体，具有明显的孔道结构。经计算CPT的载药量为 (1.80 ± 0.23) %。CPT@PB NPs具有良好的光热性能，经计算光热效率为η为35.42%。CCK-8实验表明，激光照射下低浓度CPT@PB NPs即对4T1细胞表现出良好的杀伤作用，且PB NPs和CPT在抗肿瘤过程中发挥协同增效的作用。结论：本研究成功的制备了CPT@PB NPs，构建了光热疗法和化疗协同作用的纳米给药系统，为乳腺癌的治疗提供了新的方法

Abstract:

OBJECTIVE The aim was to synthesize Cryptotanshinone-loaed Prussian Blue Nanoparticles (CPT@PB NPs) and to evaluate the physicochemical properties, photothermal performance, and cytotoxicity of the prepared nanoparticles. METHODS The CPT@PB NPs were fabricated using a hydrothermal synthesis method coupled with a solvent adsorption technique. The nanoparticles were characterized by Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS). The photothermal effect of the nanoparticles was assessed using an infrared thermal imaging camera. Finally, the in vitro anti-tumor activity of CPT@PB NPs was evaluated through the CCK-8 assay. RESULTS The synthesized CPT@PB NPs presented a regular cubic morphology with distinct porous structures. The drug loading capacity of CPT had been calculated to be (1.80 ± 0.23)%. The CPT@PB NPs exhibited excellent photothermal properties with a calculated photothermal conversion efficiency (η) of 35.42%. The CCK-8 assay indicated that low concentration CPT@PB NPs had a significant cytotoxic effect on 4T1 cells under laser irradiation, and the PB NPs and CPT synergistically enhanced the anti-tumor effects. CONCLUSION This study successfully synthesized CPT@PB NPs, establishing a nanocarrier system that synergized photothermal therapy with chemotherapy. This provided a novel approach for the treatment of breast cancer.