黄精多糖的制备及延缓皮肤衰老活性研究

Study on the Preparation and Delay Skin Aging Activity of Polygonatum Polysaccharide

关键词:  黄精多糖  提取工艺  单糖组成  抗氧化  延缓皮肤衰老

Key words:  Polygonatum polysaccharide  Extraction process  Monosaccharide composition  Antioxidant  Delay skin aging

摘要:

目的 制备多花和长梗两种来源的黄精多糖,优化提取工艺,对其进行理化性质分析并评价其延缓皮肤衰老的活性。方法 采用水提醇沉法,以提取温度、料液比、提取时间为考察因素,采用单因素及正交试验优化黄精多糖的提取条件,通过除蛋白和透析纯化多糖,采用高效液相色谱法(HPLC)和傅里叶红外光谱法(FT-IR)分析单糖组成及其结构,并结合体外自由基清除试验和H2O2诱导人皮肤成纤维细胞(HFF-1)衰老试验评价其抗氧化和延缓皮肤衰老的活性。结果 黄精多糖的最佳提取工艺为提取温度80℃,料液比为1:25(g:mL),提取时间1.0h。多花黄精多糖(PCP)和长梗黄精多糖(PFP)均含D-甘露糖、D-半乳糖醛酸、D-(+)-无水葡萄糖、D-半乳糖和DL-阿拉伯糖等5种单糖,而PFP含特征单糖鼠李糖；PCP中D-甘露糖和D-(+)-无水葡萄糖摩尔百分比较高,而PFP中D-甘露糖、D-半乳糖和D-半乳糖醛酸摩尔百分比较高,且均高于PFP。PCP中含有α和β两种构型的糖苷键,PFP中糖醛酸的特征显著。PCP和PFP均具有较好的自由基清除能力和延缓皮肤细胞衰老的作用,且PFP的活性更强。结论 本实验通过工艺优化获得了单糖组成和含量均有所不同的PCP和PFP,两者均具有较好的延缓皮肤衰老活性,该研究结果可为黄精多糖在抗衰老化妆品中的开发应用提供科学依据。

Abstract:

ABSTRACT: OBJECTIVE To prepare polysaccharides from two sources of Polygonum cyrtonema Hua and Polygonatum filipes Merr, the extraction process was optimized. The physical and chemical properties were analyzed and the activity of delaying skin aging was evaluated. METHODS The water extraction and alcohol precipitation method was used to optimize the extraction conditions of polysaccharides by single factor and orthogonal test with extraction temperature, solid-liquid ratio and extraction time as the investigation factors. The polysaccharides were purified by protein removal and dialysis. The monosaccharide composition and structure were analyzed by HPLC and FT-IR. The antioxidant and delay skin aging activities of PCP and PFP were evaluated by in vitro free radical scavenging test and H2O2-induced human skin fibroblast (HFF-1) senescence test. RESULTS The optimum extraction conditions were as follows : extraction temperature 80°C, solid-liquid ratio 1:25 (g : mL), extraction time 1.0h. PCP and PFP both contained D-Mannose, D-Galacturonic acid monohydrate, D-(+)-Glucose, D-Galactose and DL-Arabinose, while PFP contained characteristic monosaccharide Rhamnose. The molar percentages of D-Mannose and D-(+)-Glucose in PCP were higher, while the molar percentages of D-Mannose, D-Galactose and D-Galacturonic acid monohydrate in PFP were higher than those in PCP . PCP contains two kinds of glycosidic bonds of α and β configurations, and the characteristics of uronic acid in PFP was significant. PCP and PFP showed good free radical scavenging ability and delay aging of skin cells, and the activity of PFP was stronger. CONCLUSION In this experiment, PCP and PFP with different monosaccharide composition and content were obtained by process optimization. Both of them have good delay skin aging activity. The results of this study could provide a scientific basis for the development and application of Polygonatum polysaccharide in anti-aging cosmetics.