| 引用本文: | 刘德穗,张军锋,马青云,杨理,谢晴宜,吴友根,赵友兴.油茶内生真菌Diaporthe arengae M2次级代谢物抑菌活性研究[J].中国现代应用药学,2025,42(23):103-109. |
| liu de sui,zhang jun feng,ma qing yun,yang li,xie qing yi,wu you gen,zhao you xing.Study on antibacterial activity of secondary metabolites of endophytic fungus Diaporthe arengae M2 in Camellia oleifolia[J].Chin J Mod Appl Pharm(中国现代应用药学),2025,42(23):103-109. |
|
| 摘要: |
| 目的? 从油茶内生真菌Diaporthe arengae M2的次级代谢物中寻找抑菌活性先导化合物。方法? 采用传统柱层析法以及semi-prep HPLC对D. arengae M2次级代谢物进行分离纯化,再通过NMR、IR、HR-ESI-MS等波谱仪器鉴定化合物结构,最后使用肉汤稀释法对这些化合物进行抑菌活性评价。结果? 从D. arengae M2的次级代谢物中分离鉴定了8个化合物:xylariphthalide A (1)、cytosporone B (2)、cytosporone E (3)、cytosporone C (4)、pestalotiopsone A (5)、pestalotiopsone C (6)、ergosterol (7)、7, 8-dimethyl-3, 10-dimethyl-iso-alloxazine (8)。抑菌活性评价显示,化合物3对3株革兰氏阳性菌的MIC值均为2 μg/mL,对E.coli与P. aeruginosa的MIC值分别为32与64 μg/mL；化合物2对4株尖孢镰刀菌的MIC值的范围在8 – 16 μg/mL之间；化合物4可以抑制S. aureus、MRSA与B.subtilis,MIC范围在8 - 16 μg/mL之间。结论? 化合物2,3和4具有良好的抑菌活性。 |
| 关键词: 油茶 内生真菌 间座壳属真菌 次级代谢物 抑菌活性 |
| DOI： |
| 分类号: |
| 基金项目:海南省自然科学基金(221MS0762)；国家中药材产业技术体系专项(CARS-21)；海南省重点研发计划项目(ZDYF2022SHFZ020) |
|
| Study on antibacterial activity of secondary metabolites of endophytic fungus Diaporthe arengae M2 in Camellia oleifolia |
|
liu de sui1, zhang jun feng1, ma qing yun2, yang li2, xie qing yi2, wu you gen3, zhao you xing2
|
|
1.School of Chemistry and Chemical Engineering，Hainan University;2.Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences;3.Nanfan Research Institute of Hainan University
|
| Abstract: |
| ABSTRACT: OBJECTIVE To find lead compounds of antibacterial activity from secondary metabolites of endophytic fungus Diaporthe arengae M2 in Camellia oleifolia. METHODS The secondary metabolites of D. arengae M2 were isolated and purified by traditional column chromatography and semi-prep HPLC. The structure of the compounds was identified by NMR, IR, HR-ESI-MS and other spectroscopic instruments. Finally, the antibacterial activity of these compounds was evaluated by broth dilution method. RESULTS A total of 8 compounds were isolated and identified from the secondary metabolites of D. arengae M2: xylariphthalide A (1), cytosporone B (2), cytosporone E (3), cytosporone C (4), pestalotiopsone A (5), pestalotiopsone C (6), ergosterol (7), 7, 8-dimethyl-3, 10-dimethyl-iso-alloxazine (8). The antibacterial activity evaluation showed that the MIC values of compound 3 against the three Gram-positive strains were 2 μg/mL, and the MIC values against E.coli and P. aeruginosa were 32 and 64 μg/mL respectively. The MIC values of compound 2 against four strains of Fusarium oxysporum ranged from 8 to 16 μg/mL. compound 4 against S. aureus, MRSA and B.subtilis with MIC ranged from 8 to 16 μg/mL. CONCLUSION Compounds 2, 3 and 4 showed better antibacterial activity. |
| Key words: Camellia oleifolia endophytic fungus Diaporthe sp. secondary metabolites antibacterial activity |
