适用于LC–MS的衍生化技术研究及应用进展

Research and Application Progresses in Derivatization Techniques for LC–MS

关键词:  液相色谱-质谱联用  药物分析  化学衍生化  稳定同位素标记  酶法衍生化

Key words:  LC–MS  pharmaceutical analysis  chemical derivatization  stable isotope labeling  enzymatic derivatization

摘要:

衍生化技术通过将目标分析物转化为易于分离和电离的衍生物,可以显著提高复杂基质中痕量化合物检测的分离度、灵敏度和(或)选择性,目前在LC–MS分析中已得到了广泛的应用。通过广泛查阅各类相关文献,本文系统总结了各类衍生化技术的基本原理和优势,综述了不同类型的化学衍生化反应及其在食品、药物、生命科学和环境分析等领域的应用进展。此外,本文还归纳了稳定同位素标记衍生化技术与酶法衍生化技术的研究进展。本文将为衍生化LC–MS技术的进一步发展和应用提供参考,为中药、生物样品等复杂样品的精准分析提供借鉴。

Abstract:

Derivatization significantly enhances the sensitivity and selectivity of LC–MS for the trace compounds detection in complex matrices by converting the target analytes into derivatives that are more easily for separation and ionization. This review introduces the fundamental principles and advantages of derivatization, as well as its applications in sample pretreatment. This review also summarizes the different types of chemical derivatization reactions and their specific applications in fields such as pharmaceuticals, food, life sciences, and environmental analysis. Additionally, the advancements in stable isotope labeling derivatization and enzymatic derivatization are explored through emphasizing the importance of derivatization in enhancing the sensitivity, selectivity, and accuracy of LC–MS analysis. Overall, derivatization has become a significant branch in the field of LC-MS analysis, demonstrating broad application prospects.