月见草油对PCOS模型大鼠NLRP3炎症体通路的影响

Effects of evening primrose oil on NLRP3 inflammatory pathway in PCOS model rats

关键词:  多囊卵巢综合症  月见草油  PCOS  大鼠  NLRP3炎症体

Key words:  polycystic ovary syndrome  Evening primrose oil  PCOS  Rat  NLRP3 inflammator

摘要:

目的 探讨月见草油(EPO)对PCOS模型大鼠NLRP3炎症体通路的改善作用及机制。方法 6周龄SD大鼠,连续21天灌胃来曲唑联合高脂饲料喂养,建立PCOS模型。成模后给予不同剂量的EPO,期间测体质量、空腹血糖(FPG)及动情周期等。取完材后ELISA法测定空腹胰岛素(FINS)、促黄体生成素(LH)等激素水平；TUNEL法检测大鼠卵巢颗粒细胞凋亡情况；HE染色法观察卵巢形态学变化；Western blotting和免疫组织化学染色法检测卵巢组织NLRP3、ASC、TNF-α、IL-18等蛋白。结果 与PCOS模型组比较,EPO干预后,PCOS大鼠体质量、FPG及LH等水平显著改善(P＜0.05),卵巢多囊病变减轻,NLRP3、ASC、TNF-α、IL-18等炎症因子表达水平均明显降低,差异有统计学意义。结论 EPO可以改善PCOS大鼠卵巢组织形态,降低FPG以及血清中FINS、LH等激素水平,同时可能通过调控NLRP3炎症体通路改善PCOS慢性炎症。

Abstract:

Objective To investigate the effect and mechanism of evening primrose oil on improving NLRP3 inflammatory pathway in PCOS model rats. Methods 6-week-old SD rats were fed with letrozole combined with high-fat diet for 21 days to establish PCOS model. After molding, different doses of evening primrose oil were given, during which body mass, fasting blood glucose (FPG) and estrus cycle were measured. The levels of fasting insulin (FINS), luteinizing hormone (LH) and other hormones were determined by ELISA. The apoptosis of ovarian granulosa cells was detected by TUNEL method. The morphological changes of ovary were observed by HE staining. Western blotting and immunohistochemical staining were used to detect NLRP3, ASC, TNF-α, IL-18 and other proteins in ovarian tissue. Results Compared with PCOS model group, after intervention with evening primrose oil, body weight, FPG and LH levels of PCOS rats were significantly improved (P < 0.05), polycystic ovarian disease was alleviated, and the expression levels of NLRP3, ASC, TNF-α, IL-18 and other inflammatory factors were significantly decreased, with statistical significance. Conclusion Evening primrose oil can improve the ovarian tissue morphology of PCOS rats, reduce the levels of FPG, FINS, LH and other hormones in serum, and may improve the chronic inflammation of PCOS by regulating the NLRP3 inflammatory pathway, and small and medium doses of evening primrose oil have the best therapeutic effect.