基于谱效关系及成分敲除的水提竹沥抗炎关键成分及其质量评价研究

Study on the key anti-inflammatory components and quality evaluation of Aqueous bamboo juice based on spectrum-effect relationship and component knockout

关键词:  竹沥  急性咽炎  指纹图谱  谱效关系  敲除  对香豆酸  (+)-南烛木树脂酚-9’-O-葡萄糖甙

Key words:  aqueous bamboo extract  Acute Pharyngitis  Fingerprint Profile  Spectrum-Effect Relationship  Knockout  p-Coumaric acid  (+)-Lyoniresinol 9'-O-glucoside  (+)-Lyoniresinol 9"-O-glucoside

摘要:

目的 建立水提竹沥指纹图谱，研究探讨水提竹沥抗炎活性谱效关系，筛选水提竹沥抗咽炎关键成分。方法 采用HPLC法建立15批同属不同种水提竹沥指纹图谱。建立LPS诱导的RAW264.7细胞炎症模型，以NO抑制率为指标，利用灰色关联度和偏最小二乘法构建谱效关系。利用成分敲除技术敲除对香豆酸，并建立急性咽炎大鼠模型，以血清中IL-1β、IL-6和TNF-α水平评价对香豆酸抗咽炎药效。结果 刚竹属水提竹沥指纹图谱共有峰有15个，通过标准品比对指认出对香豆酸和 (+)-南烛木树脂酚-9’-O-葡萄糖苷2个成分，不同属水提竹沥成分差异较大，其中对香豆酸成分是区分的重要指标；谱效研究结果表明色谱峰7、8、9（对香豆酸）、10((+)-南烛木树脂酚-9’-O-葡萄糖苷)可能是水提竹沥发挥抗炎活性的重要成分。基于成分敲除法验证了对香豆酸能显著降低急性咽炎大鼠血清中IL-1β、IL-6及TNF-α水平，是抗咽炎重要成分。结论 建立的水提竹沥指纹图谱可用于水提竹沥的质量评价，谱效关系研究初步阐明了水提竹沥抗炎物质基础，结合成分敲除法确定了对香豆酸为其抗咽炎关键成分之一，为水提竹沥的质量控制与临床应用提供参考。

Abstract:

OBJECTIVE To establish the fingerprint profiles of aqueous bamboo extract from different species of the same genus, explore the anti-inflammatory activity-spectrum relationship, and screen for key components with anti-pharyngitis properties. METHODS HPLC was employed to establish the fingerprint profiles of 15 batches of bamboo extract from different species. An LPS-induced inflammation model in RAW 264.7 cells was used, with NO inhibition rate as an indicator. Spectrum-effect relationships were constructed using grey correlation analysis and partial least squares regression. The therapeutic efficacy of p-coumaric acid in the treatment of acute pharyngitis inrats was confirmed using the component knockout method, resulting in a significant reduction in serum levels of IL-1β, IL-6, and TNF-α. RESULTS The fingerprint profiles of aqueous bamboo extract revealed 15 common peaks, with p-coumaric acid and (+)-liriodendrin-9’-O-glucoside identified by comparison with reference standards. Spectral-activity relationship analysis indicated that chromatographic peaks 7, 8, and 9 (coumaric acid), as well as peak 10 ((+)-Lyoniresinol 9'-O-glucoside), might be critical contributors to the anti-inflammatory activity of aqueous bamboo extract. It was verified that p-coumaric acid can significantly reduce the levels of IL-1β, IL-6 and TNF-α in serum of rats with acute pharyngitis, and is an important anti-pharyngitis component. CONCLUSION The established fingerprint profile can be used to evaluate the quality of aqueous bamboo extract. The spectrum-effect relationship study preliminarily elucidated the anti-inflammatory active substances of aqueous bamboo extract, and component knockout experiments identified p-coumaric acid as one of the key components for its anti-pharyngitis effect, providing a reference for quality control and clinical application of aqueous bamboo extract.