| 引用本文: | 陈金妹,潘轶,徐金铭,吴会晗,俞冰,张水利.基于网络药理学和动物实验研究酒黄精抗肝纤维化的作用机制[J].中国现代应用药学,2026,43(10):10-22. |
| chen jinmei,Pan Yi,Xu Jinming,Wu Huihan,Yu Bing,Zhang Shuili.Study on the mechanism of wine-processed Polygonati Rhizoma anti liver fibrosis effect based on network pharmacology and animal experiments[J].Chin J Mod Appl Pharm(中国现代应用药学),2026,43(10):10-22. |
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| 基于网络药理学和动物实验研究酒黄精抗肝纤维化的作用机制 |
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陈金妹,潘轶,徐金铭,吴会晗,俞冰,张水利
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1.浙江中医药大学附属第二医院;2.浙江中医药大学;3.浙江中医药大学附属第三医院;4.浙江中医药大学金华研究院
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| 摘要: |
| 目的 探索酒黄精抗肝纤维化的作用机制。方法 利用液质联用技术检测并分析酒黄精化学成分,采用网络药理学预测酒黄精可能的生物学靶点以及潜在的信号通路。对筛选出的活性成分与肝纤维化关键靶点进行分子对接验证,建立四氯化碳(CCl4)诱导大鼠肝纤维化模型,通过肝脏表型分析、肝组织病理学分析、生化分析等一系列指标测定酒黄精的抗肝纤维化效果,使用WB进一步验证网络药理学预测的途径。结果 共分析鉴定28个化学成分,网络药理学研究共筛选出活性成分17个,交集靶点235个,PI3K/Akt信号通路可能是酒黄精抗肝纤维化作用的主要通路之一。动物实验表明,酒黄精可以缓解CCl4诱导的肝纤维化,并且显著降低了PI3K/Akt信号通路中蛋白靶标的表达。结论 酒黄精对CCl4诱导的肝纤维化具有保护作用,其通过多成分、多靶点、多通路发挥效应,其保护机制可能与抑制PI3K/Akt信号通路有关。 |
| 关键词: 酒黄精 肝纤维化 网络药理 PI3K/Akt信号通路 |
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| Study on the mechanism of wine-processed Polygonati Rhizoma anti liver fibrosis effect based on network pharmacology and animal experiments |
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chen jinmei1,2,3,4,5,6,7,8,9, Pan Yi6,7,8,9, Xu Jinming1,10,3,4,5,6,7,8,9, Wu Huihan11,6,7,8,9, Yu Bing6,7,8,9, Zhang Shuili6,7,8,9
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1.The 2.Second 3.Affiliated 4.Hospital 5.of 6.Zhejiang 7.Chinese 8.Medical 9.University;10.Third 11.Jinhua Research Institute,
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| Abstract: |
| Objective To explore the anti-hepatic fibrosis mechanism of wine-processed Polygonati Rhizoma. Methods The chemical constituents of wine-processed Polygonati Rhizoma were detected and analyzed by LC-MS, and the compound target network of liver fibrosis was constructed by combining with network pharmacology to predict the possible biological targets and potential signal pathways of wine-processed Polygonati Rhizoma. A rat model of hepatic fibrosis induced by carbon tetrachloride (CCl4) was established. The anti-hepatic fibrosis effect of wine-processed Polygonati Rhizoma was determined by a series of indexes such as liver phenotype analysis, liver histopathology analysis and biochemical index analysis, and the way of network pharmacology prediction was further verified by protein blot analysis. Results Twenty-eight chemical constituents from wine-processed Polygonati Rhizoma were identified, and 235 intersecting targets of anti-hepatic fibrosis of wine-processed Polygonati Rhizoma were obtained by network phar |
| Key words: wine-processed Polygonati Rhizoma Hepatic fibrosis Network pharmacology PI3K/Akt pathway |
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