| 引用本文: | 贺木兰,王思思,金原震,王志强,邹妍,李文龙,李力,向铮,瞿海斌.瓜蒌薤白半夏汤化学成分的LC-MS定性定量分析[J].中国现代应用药学,2026,43(9):17-23. |
| HE Mulan,WANG Sisi,JIN Yuanzhen,WANG Zhiqiang,ZOU Yan,LI Wenlong,LI Li,XIANG Zheng,QU Haibin.Qualitative and quantitative analysis of chemical constituents of Gualou-Xiebai-Banxia Decoction based on LC-MS[J].Chin J Mod Appl Pharm(中国现代应用药学),2026,43(9):17-23. |
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| 瓜蒌薤白半夏汤化学成分的LC-MS定性定量分析 |
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贺木兰1, 王思思2, 金原震2, 王志强3, 邹妍3, 李文龙1, 李力4, 向铮2, 瞿海斌1
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1.天津中医药大学;2.浙大城市学院;3.神威药业集团有限公司;4.浙江医院
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| 摘要: |
| 目的 对瓜蒌薤白半夏汤的化学成分进行定性分析,并对其中9种成分进行定量测定。方法 采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Orbitrap-MS)对瓜蒌薤白半夏汤的化学成分进行分析,通过中药标品数据库LuMet-TCM及Herb数据库对化合物进行结构鉴定。同时建立高效液相色谱-串联质谱(HPLC-MS/MS)同时测定该汤剂中香叶木素、知母皂苷BII、琥珀酸、烟酰胺、葫芦巴碱、腺苷、5-羟甲基糠醛、鸟苷、薤白苷含量的方法,并进行精密度、重复性、稳定性、线性关系、加样回收率考察。结果 汤剂中共鉴定出256个成分,包括氨基酸及多肽类、黄酮类、苯丙素类、生物碱类等化合物,同时定量瓜蒌薤白半夏汤中9种成分的方法,其中5-羟甲基糠醛、琥珀酸、腺苷、薤白苷等含量较高,分别为540.13、521.98、21.80、23.40 μg/mL。结论 本研究为瓜蒌薤白半夏汤的药效物质筛选和质量控制奠定了基础。 |
| 关键词: 瓜蒌薤白半夏汤 化学成分 定性定量分析 |
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| 基金项目:浙江省“尖兵领雁+X”科技计划项目 |
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| Qualitative and quantitative analysis of chemical constituents of Gualou-Xiebai-Banxia Decoction based on LC-MS |
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HE Mulan1, WANG Sisi2, JIN Yuanzhen2, WANG Zhiqiang3, ZOU Yan3, LI Wenlong1, LI Li4, XIANG Zheng2, QU Haibin1
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1.Tianjin University of Traditional Chinese Medicine;2.Hangzhou City University;3.Shenwei Pharmaceutical Group Co., Ltd;4.Zhejiang Hospital
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| Abstract: |
| ABSTRACT: OBJECTIVE To qualitatively analyze the chemical components of Gualou-Xiebai-Banxia Decoction and determine the contents of 9 components. METHODS The chemical composition of Gualou-Xiebai-Banxia Decoction was analyzed using UPLC-Q-Orbitrap-MS, and the compounds were structurally identified using the traditional Chinese medicine standard database LuMet-TCM and Herb database. A HPLC-MS/MS was established to simultaneously diosmetin, timosaponin BII, succinic acid, nicotinamide, trigonelline, adenosine, 5-hydroxymethylfurfural, guanosine and β-D-galactopyranoside content in this decoction. The method was used to investigate the precision, repeatability, stability, linear relationship, and sample recovery. RESULTS A total of 256 components were identified in the decoction, including amino acids and polypeptides, flavonoids, phenylpropanoids, alkaloids and other compounds. At the same time, 9 components in Gualou-Xiebai-Banxia Decoction were quantified, among which the contents of 5-hydroxymethylfurfural, succinic acid, adenosine and (25R)-26-O-β-D-glucopyranosyl-22-hydroxy-5β-furost-3β,26-diol-3-O-β-D-glucopyranosyl-(1→2)-β-D-galactopyranoside were higher, which were 540.13, 521.98, 21.80 and 23.40 μg/mL, respectively. CONCLUSION This study laid a foundation for the screening and quality control of pharmacodynamic substances of Gualou-Xiebai-Banxia Decoction. |
| Key words: gualou-xiebai-banxia decoction, chemical composition, qualitative and quantitative analysis |
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