洋葱伯克霍尔德氏菌群生长建模研究

Growth Modelling of Burkholderia cepacia complex

关键词:  洋葱伯克霍尔德菌群  生长动力学  一级模型  二级模型  药品安全

Key words:  Burkholderia cepacia complex  growth kinetics  primary model  secondary model  pharmaceutical safety

摘要:

目的 探究营养浓度及温度条件对洋葱伯克霍尔德菌群(Burkholderia cepacia Complex, Bcc)典型菌株生长动力学的影响,并采用预测微生物学方法预测其生长风险。方法 通过Baranyi模型和主参数模型对不同营养浓度和温度条件下Bcc菌株的生长动力学参数进行估计和比较。结果 营养浓度与最大菌浓度呈正相关(TSB:9.80~10.08 log CFU/mL；1/100 TSB:9.01~9.21 log CFU/mL),但未显著改变最大比生长速率和延滞期,证实Bcc具有寡营养耐受特性。温度显著影响菌群生长动力学参数,其适宜生长范围为20~40 ℃,其中多噬伯克霍尔德菌(B. multivorans)表现出更高的最适比生长速率和更低的温度生长阈值。结论 该研究为定量预测和评估Bcc在药品生产及贮藏环境中的生长风险提供了关键动力学参数,对优化其污染防控策略具有重要参考意义。

Abstract:

OBJECTIVE To investigate the effects of nutrient concentration and temperature on growth kinetics of type strains in the?Burkholderia cepacia?complex (Bcc), and evaluate their proliferation risks through predictive microbiology. METHODS Growth kinetic parameters under different nutrient conditions and temperature regimes were estimated using Baranyi and primary models. Comparative analyses were conducted among?B. multivorans,?B. cepacia, and?B. cenocepacia. RESULTS Nutrient concentration positively correlated with maximum population density (TSB: 9.80-10.08 log CFU/mL; 1/100 TSB: 9.01-9.21 log CFU/mL), whereas maximum specific growth rate (μmax) and lag phase duration showed no significant variation, demonstrating Bcc""s oligotrophic tolerance. Temperature critically regulated growth kinetics within 20-40 °C.?B. multivorans?exhibited higher μmax?and lower minimum growth temperature compared to?B. cepacia?and?B. cenocepacia. CONCLUSION This study provides essential kinetic parameters for predicting Bcc proliferation risks in pharmaceutical manufacturing and storage, offering critical evidence for optimizing contamination control strategies.