小麦胚芽肽和獐牙菜苦苷对UVB诱导的小鼠日光性皮炎的协同保护作用研究

Study on the Synergistic Protective Effects of Wheat Germ Peptides and Swertia Bitter Glycosides against UVB-induced Solar Dermatitis in MiceMA Yuewen1,2,3, TAO Qinqian4,ZHU Chaofan4,YAN Erping4*,CHEN Xian5, WANG Chunli1,2,3*

关键词:  小麦胚芽肽  獐牙菜苦苷  紫外线B  日光性皮炎  抗氧化  抗炎  协同作用

Key words:  wheat germ peptide  Swertiamarin  ultraviolet B  solar dermatitis  antioxidant  anti-inflammatory  cooperative interaction

摘要:

目的：研究小麦胚芽肽和獐牙菜苦苷对紫外线B(UVB)诱导的日光性皮炎小鼠的协同保护作用。方法：将KM小鼠随机分为6组(n=12)：空白组(Blank)、模型组(Model)、康复新液组(KFXY)、40 mg.kg-1^{小麦胚芽肽组}(DSM1),40 mg.kg-1^{獐牙菜苦苷组}(DSM2),20 mg.kg-1^{小麦胚芽肽}+20 mg.kg-1^{獐牙菜苦苷组}(DSM3)。空白组小鼠仅进行剃毛,不照射,其余组进行UVB照射。给药组均外用给药,共干预7天。实验结束后,拍照记录小鼠的皮肤状态,对皮肤组织进行苏木精-伊红(HE)染色、苦味酸-酸性品红法(VG)染色。通过生化检测皮肤组织中氧化应激指标T-AOC、NOS、NO、MDA的含量及活力水平,通过免疫组化检测皮肤组织中炎症指标TNF-α、IL-6、IL-1β水平,通过qRT-PCR检测皮肤组织中NQO1、GCLC、Nrf2的mRNA水平,通过Western blotting检测皮肤组织中NQO1、GCLC、Nrf2的蛋白水平。结果：与UVB模型组对比,DSM1、DSM2、DSM3组小鼠皮肤损伤程度有所改善,胶原纤维排列整齐,胶原密度升高,T-AOC水平提高,NOS、NO、MDA水平降低,TNF-α、IL-6、IL-1β 表达水平降低,皮肤组织中NQO1、GCLC、Nrf2的mRNA表达水平升高,NQO1、GCLC、Nrf2的蛋白相对表达量升高。结论：小麦胚芽肽、獐牙菜苦苷联用均可改善UVB介导的小鼠日光性皮炎,推测其作用机制与Nrf2信号通路有关。

Abstract:

［ABSTRACT］ OBJECTIVE：To study the synergistic protective effects of wheat germ peptides and swertia bitter glycosides in mice with ultraviolet B (UVB)-induced solar dermatitis. METHODS：KM mice were randomly divided into 6 groups (n=12): Blank group(Blank), Model group(Model), rehabilitated new group (KFXY), 40 mg·kg-1 wheat germ peptide group (DSM1), 40 mg·kg-1 swertiamarin group (DSM2), and 20 mg·kg-1 wheat germ peptide+20 mg·kg-1 swertiamarin group (DSM3). Mice in the blank group were only shaved and not irradiated, while the remaining groups were irradiated with UVB. The drug delivery groups were treated for 7 days. At the end of the experiment, photos were taken to record the skin status of the mice, and the skin tissues were stained with HE staining, picric acid-acidic magenta method (VG) staining. The content and viability levels of oxidative stress indicators T-AOC, NOS, NO and MDA in skin tissues were detected by biochemistry, the levels of inflammatory indicators TNF-α, IL-6 and IL-1β in skin tissues were detected by immunohistochemistry, the mRNA levels of NQO1, GCLC and Nrf2 in skin tissues were detected by qRT-PCR. The protein levels of NQO1, GCLC and Nrf2 in skin tissues were detected by Western blotting. RESULTS：Compared with the UVB group, the degree of skin damage in the DSM1, DSM2 and DSM3 groups was improved, the collagen fibers were neatly arranged, the collagen density increased, the levels of T-AOC increased, the levels of NOS, NO and MDA decreased, the expression levels of TNF-α, IL-6 and IL-1β decreased, the mRNA expression levels of NQO1, GCLC and Nrf2 increased, the relative expression levels of NQO1, GCLC and Nrf2 were increased. CONCLUSION：Wheat germ peptide and swertia bitter glycosides alone and in combination ameliorated UVB-mediated solar dermatitis in mice, which may be related to Nrf2 pathways.