| 引用本文: | 张毕杰,苏丹,宋永贵,刘亚丽,平欲晖,朱根华,郑琴,阳明,艾志福.基于UPLC-Q-TOF-MS/MS技术的经典方抑肝散化学成分及其血、脑移行成分分析[J].中国现代应用药学,2026,43(3):83-94. |
| ZHANG Bijie,SU Dan,SONG Yonggui,LIU Yali,PING Yuhui,ZHU Genhua,ZHENG Qin,YANG Ming,AI Zhifu.Analysis of the chemical constituents of the classical formula Yigan San and its blood and brain migratory components based on UPLC-Q-TOF-MS/MS technology[J].Chin J Mod Appl Pharm(中国现代应用药学),2026,43(3):83-94. |
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| 基于UPLC-Q-TOF-MS/MS技术的经典方抑肝散化学成分及其血、脑移行成分分析 |
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张毕杰1, 苏丹1, 宋永贵1, 刘亚丽2, 平欲晖1, 朱根华1, 郑琴1, 阳明1, 艾志福1
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1.江西中医药大学;2.南昌医学院
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| 摘要: |
| [摘要]：目的：建立一种快速鉴别中药经典方抑肝散化学成分的有效方法，并探索其口服给药后在血液和脑组织中的移行成分。方法：采用超高效液相色谱-四极杆-飞行时间串联质谱法（UPLC-Q-TOF-MS/MS）进行数据采集。根据化合物保留时间、准分子离子峰、二级碎片离子等信息，对抑肝散水提物及口服后的血液和脑组织中的移行成分进行表征与鉴定。结果：从抑肝散水提物中共鉴定出170个化合物，其中包括黄酮类47个、其他类32个、内酯类26个、有机酸类24个、萜类15个、生物碱类11个、香豆素类8个、皂苷类7个。从血液中共鉴定出31个化合物，从脑组织中共鉴定出8个化合物；此外，还发现13个代谢产物，涵盖还原、氧化、羟基化、葡萄糖醛酸化等I、II相代谢过程。结论：本研究高通量分析了抑肝散的化学成分，揭示了其口服给药后在血液和脑组织中的传递，其中东莨菪素、洋川芎内酯、刺芒柄花素可能是其潜在药效物质基础，可为今后该复方的质量标准提升、药效物质研究，以及活性药物开发提供科学依据。 |
| 关键词: UPLC-Q-TOF-MS/MS 抑肝散 化学成分 入血成分 入脑成分 裂解规律 |
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| 基金项目:国家中医药多学科交叉创新团队项目（ZYYCXTD-D-202207）；江西省杰青需求牵引类项目（20224ACB218008）；江西中医药大学科技创新团队（CXTD22008） |
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| Analysis of the chemical constituents of the classical formula Yigan San and its blood and brain migratory components based on UPLC-Q-TOF-MS/MS technology |
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ZHANG Bijie1, SU Dan1, SONG Yonggui1, LIU Yali2, PING Yuhui1, ZHU Genhua1, ZHENG Qin1, YANG Ming1, AI Zhifu1
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1.Key Research Room for Chinese Medicine Efficacy Evaluation Prevention and Treatment of Mental Disorders Brain Disease of Jiangxi Traditional Chinese Medicine Administration/Key Research Room for Depression Syndrome Animal Models of Jiangxi Traditional Chinese Medicine Administration,Jiangxi University of Chinese Medicine;2.Nanchang Medical College
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| Abstract: |
| Abstracts:Objective To establish an effective method to rapidly identify the chemical components of the classic Chinese medicine prescription Yigan San, and to explore its migratory components in blood and brain tissues after oral administration.Methods Data acquisition was performed by ultra-performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS).According to the information of compound retention time, excimer ion peak, secondary fragment ion and other information, the migration components in blood and brain tissues after oral administration were characterized and identified.Results A total of 171 compounds were identified from the water extract of Yigansan, including 47 flavonoids, 32 others, 26 lactones, 24organic acids, 16 terpenoids, 11 alkaloids, 8 coumarins and 7 saponins.A total of 31 compounds were identified in the blood and 8 compounds in the brain tissue. Additionally, 13 metabolites were discovered, covering phase I and phase II metabolic processes such as reduction, oxidation, hydroxylation, and glucuronidation.Conclusion In this study, the chemical composition of Yigansan was analysed in high throughput, revealing its delivery in blood and brain tissues after oral administration, in which scopolamine, yangchuanxionglactone, and prickly mangosteen propionin may be the basis of potentialpharmacodynamic substances, which may provide a scientific basis for the improvement of the quality standard of the compound, the study of pharmacodynamic substances, and the development of the active pharmaceuticals in the future. |
| Key words: UPLC-Q-TOF-MS/MS Yigan San Chemical composition Blood components Brain-penetrating component fragmentation pattern |
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