附子粳米汤对氢化可的松致肾阳虚模型大鼠机体改善作用研究

Effect of Fuzi-jingmi decoction on hydrocortisone-induced kidney Yang deficiency model rats

关键词:  附子粳米汤  肾阳虚  行为学  血液学指标  HPA轴  肾脏水通道

Key words:  fuzi-jingmi decoction  kidney yang deficiency  behavior  hematological indexes  HPA axis  renal water channel

摘要:

目的 探讨附子粳米汤对氢化可的松致肾阳虚模型大鼠的影响。方法 采用SD大鼠每天肌注氢化可的松注射液,连续10天制备肾阳虚模型。造模同时分别灌胃给予附子粳米汤(2.0、4.0、8.0 g.kg-1),连续给药5周。实验期间检测大鼠体征指标(体重、形态学、抓力、自主活动次数、背温、面温、旷场分析、蔗糖偏好及精子质量)；检测血液学指标,血生化指标,血清激素水平(CRH、ACTH及CORT)；测定肾、睾丸、附睾和精囊湿重及系数；HE染色观察肾脏组织形态学改变；Western Blot法检测肾脏AQP1与AQP2蛋白表达；qPCR法检测肾脏AQP1与AQP2 mRNA表达。结果 ①体征指标:附子粳米汤能提高模型大鼠抓力、自主活动次数、背温、面温及蔗糖偏好率,改善脚趾肿大及精子质量；②血液学指标:升高红细胞类、白细胞类及血小板类指标,增加淋巴细胞数量及比例,降低中性粒细胞数量及比例；③血生化指标:降低血清CR、UA水平；④激素水平:升高血清CRH、ACTH及CORT激素水平；⑤脏器系数:提高肾湿重及系数；⑥组织形态学:改善肾脏组织病变；⑦肾脏水通道受体表达:增加肾脏AQP1、AQP2蛋白及mRNA表达。结论 附子粳米汤能改善氢化可的松致肾阳虚模型大鼠腰膝酸软、畏寒肢冷、性欲减退、精神萎靡等肾阳虚证候,还能改善肾脏利水功能；其机制可能与调控下丘脑-垂体-肾上腺(HPA)轴及肾脏水通道蛋白AQP1、AQP2表达有关。

Abstract:

ABSTRACT OBJECTIVE To investigate the effect of Fuzi-jingmi decoction on hydrocortisone-induced kidney-yang deficiency rats. METHODS SD rats were intramuscularly injected with hydrocortisone injection daily for 10 consecutive days to establish the model of kidney yang deficiency. During the modeling period, rats were given Aconite and Fuzi-jingmi decoction (2.0, 4.0, 8.0 g.kg-1) by gavage respectively for 5 weeks. During the experiment, the body sign indicators of rats (body weight, morphology, grip strength, spontaneous activity frequency, back temperature, face temperature, open field test, sucrose preference and sperm quality) were detected. Blood was collected to detect hematological indicators, blood biochemical indicators, and serum hormone levels (CRH, ACTH and CORT). The wet weight and coefficient of the kidney, testis, epididymis and seminal vesicle were measured. HE staining was used to observe the morphological changes of the kidney tissue. Western Blot was used to detect the expression of AQP1 and AQP2 proteins in the kidney. qPCR was used to detect the expression of AQP1 and AQP2 mRNA in the kidney. RESULTS ①Physical sign indicators: Aconite and Fuzi-jingmi Decoction can increase the grasping force, spontaneous activity frequency, back temperature, facial temperature and sucrose preference rate of model rats, improve toe swelling and sperm quality; ②Hematological indicators: increase red blood cell, white blood cell and platelet indicators, increase the number and proportion of lymphocytes, and decrease the number and proporti；on of neutrophils; ③Blood biochemical indicators: reduce serum CR and UA levels; ④Hormone levels: increase serum CRH, ACTH and CORT hormone levels; ⑤ coefficient of organs: increase the coefficient of the kidney; ⑥Histomorphology: improve renal tissue lesions; ⑦Expression of renal aquaporin receptors: increase the expression of AQP1 and AQP2 protein and mRNA in the kidney. CONCLUSION Fuzi-jingmi decoction can improve the kidney Yang deficiency syndrome of hydrocortisone-induced kidney Yang deficiency rats, such as soreness and weakness of waist and knee, cold and cold limbs, decreased libido, and lethargy, and can also improve the kidney water-promoting function. The mechanism may be related to the regulation of hypothalamic-pituitary-adrenal (HPA) axis and the expression of aquaporins AQP1 and AQP2 in kidney.