基于UPLC-MS/MS与网络药理学的寿星补汁口服液抗衰老活性成分系统解析

Systematic Characterization of Anti-Aging Active Constituents in Shouxing Buzhi Oral Liquid Based on UPLC-MS/MS and Network Pharmacology

关键词:  寿星补汁  UPLC-MS/MS  抗衰老  网络药理学

Key words:  Shouxing Buzhi  UPLC-MS/MS  aging  network pharmacology

摘要:

目的：系统解析寿星补汁口服液的化学成分，挖掘其抗衰老活性成分并探究可能的作用机制。方法：采用UPLC-Q-Tof MS/MS技术鉴定口服液中的化学成分；通过SEA、SwissTargetPrediction、Genecards等数据库检索寿星补汁口服液活性成分和抗衰老作用对应的靶点；使用String数据库构建靶点蛋白相互作用网络，并根据拓扑学参数评估靶点重要性；使用DAVID数据库进行GO功能与KEGG通路富集分析；使用Cytoscape 3.8.2软件构建药材-化合物-靶点-通路复合网络图。结果：共鉴定108种化合物，涵盖莽草酸-苯丙素、萜类等6类生物合成途径成分；通过构建“成分-靶点-通路”网络，筛选出阿魏酰酪胺、6-姜酚、甘草查尔酮B等核心活性成分；富集分析表明其通过调控SRC、STAT3、EGFR等关键靶点，显著激活PI3K-Akt、HIF-1等20条通路，介导氧化应激抑制、细胞凋亡调控及胰岛素信号转导等生物学过程，揭示复方多靶点协同抗衰老的作用机制。结论：本研究初步证明了寿星补汁口服液成分可以多成分、多靶点、多通路共同作用发挥抗衰老药效，为进一步深入研究寿星补汁口服液抗衰老作用的物质基础以及作用机制提供参考。

Abstract:

ABSTRACT: OBJECTIVE To systematically analyze the chemical composition of Shouxing Buzhi Oral Liquid, identify its anti-aging active constituents, and explore potential mechanisms of action. METHODS Identified chemical components in the oral liquid using UPLC-Q-Tof MS/MS technology. Screened targets for active constituents and anti-aging effects via SEA, SwissTargetPrediction, and Genecards databases. Constructed a protein-protein interaction network using the STRING database and evaluated target significance through topological parameters. Conducted GO functional and KEGG pathway enrichment analyses using the DAVID database.Built a herb-compound-target-pathway network using Cytoscape 3.8.2 software. RESULTS 108 compounds spanning 6 biosynthetic pathways (e.g., shikimate-phenylpropanoid and terpenoid pathways) were identified. Core active constituents (Moupinamide, 6-Gingerol, Licochalcone B) were screened via the "compound-target-pathway" network. Enrichment analysis revealed significant activation of 20 pathways (PI3K-Akt, HIF-1, etc.) by regulating key targets (SRC, STAT3, EGFR). Biological processes mediated: oxidative stress inhibition, apoptosis regulation, and insulin signal transduction, demonstrating multi-target synergistic anti-aging mechanisms. CONCLUSION This study preliminarily verifies that Shouxing Buzhi Oral Liquid exerts anti-aging effects through multi-component, multi-target, and multi-pathway interactions, providing a reference for further research on its material basis and mechanisms of action against aging.