基于山药多糖对沙棘黄酮保护作用的沙棘山药胶囊工艺研究

Study on the process improvement of sea buckthorn yam capsule based on the protective effect of yam polysaccharide on sea buckthorn flavonoids

关键词:  沙棘黄酮  山药多糖  纳米颗粒  喷雾干燥

Key words:  sea buckthorn flavonoids  yam polysaccharides  nanoparticles  spray drying

摘要:

目的 研究基于中药复方配伍理论,通过解析山药与沙棘的协同作用机制,创新性地利用山药多糖构建纳米递送系统,显著提高了沙棘黄酮的生物利用度。方法 采用水提醇沉法从山药粉中精制多糖成分,并通过高速剪切乳化技术将山药多糖溶液与沙棘黄酮乙醇溶液按3:1体积比复合。经旋转蒸发浓缩后,采用喷雾干燥工艺制备获得具有核壳结构的纳米复合颗粒。结果 场发射扫描电镜(FESEM)分析显示,所得微球粒径为20-50 μm,其表面呈现典型纳米组装形貌；透射电镜(TEM)进一步证实沙棘黄酮纳米颗粒被连续相的山药多糖网络有效包覆,通过空间位阻效应显著抑制了纳米颗粒的氢键团聚现象。溶出度实验表明,经工艺优化后的胶囊制剂在模拟肠液中60 min累积溶出度达到84.06%,较原料药粉末(31.38%)提升52.68%。结论 研究创新地将传统中药“药对”配伍理论与现代纳米制剂技术相结合,不仅揭示了多糖-黄酮纳米复合体系的稳定化机制,更为中药复方制剂的现代化改造提供了具有普适性的技术范式。

Abstract:

This study is grounded in the theory of traditional Chinese medicine (TCM) compound compatibility. By elucidating the synergistic mechanism between Chinese yam and sea buckthorn, it innovatively utilizes Chinese yam polysaccharides to construct a nano-delivery system, significantly enhancing the bioavailability of sea buckthorn flavonoids. The polysaccharide components were refined from Chinese yam powder using a water extraction and alcohol precipitation method. The Chinese yam polysaccharide solution was then combined with a sea buckthorn flavonoid ethanol solution in a 3:1 volume ratio using high-speed shear emulsification technology. After concentration by rotary evaporation, spray drying was employed to prepare nano-composite particles with a core-shell structure. Field emission scanning electron microscopy (FESEM) analysis revealed that the obtained microspheres had a particle size of 20-50 μm, with their surfaces exhibiting typical nano-assembled morphologies. Transmission electron microscopy (TEM) further confirmed that the sea buckthorn flavonoid nanoparticles were effectively encapsulated within the continuous network of Chinese yam polysaccharides, significantly inhibiting the hydrogen bond aggregation of nanoparticles through steric hindrance effects. Dissolution tests showed that the optimized capsule formulation achieved a cumulative dissolution rate of 84.06% in simulated intestinal fluid within 60 minutes, a 52.68% increase compared to the raw medicinal powder (31.38%). This study innovatively integrates the traditional TCM "herb pair" compatibility theory with modern nano-formulation technology, not only revealing the stabilization mechanism of the polysaccharide-flavonoid nano-composite system but also providing a universal technical paradigm for the modernization of TCM compound formulations.