TRA-BT-FNL的制备及其对银屑病细胞模型异常增殖的影响

Preparation of TRA-BT-FNL and its effect on abnormal proliferation in psoriatic cell model

关键词:  维甲酸  倍他米松  HaCat细胞  异常增殖

Key words:  retinoic acid  betamethasone  HaCat cells  abnormal proliferation

摘要:

目的 探讨维甲酸倍他米松柔性纳米脂质体的银屑病细胞模型的协同治疗作用,为维甲酸倍他米松柔性纳米脂质体的制剂开发提供了理论依据。方法 采用ODA-FITC标记的柔性纳米脂质体考察其细胞摄取及经皮渗透情况；采用 KGF诱导HaCat细胞建立银屑病异常增殖细胞模型；采用MTT法以OD值为指标考察维甲酸倍他米松柔性纳米脂质体对细胞模型异常增殖的影响；采用酶联免疫法检测炎症因子的变化。结果 柔性纳米脂质体在HaCaT细胞上的摄取具有一定的时间依赖性,具有良好的经皮渗透性,KGF刺激HaCaT细胞24 h,浓度选择80 ng/mL可建立银屑病异常增殖细胞模型。 维甲酸倍他米松柔性纳米脂质体溶液可显著抑制HaCaT银屑病细胞模型的异常增殖,酶联免疫实验表明其可显著降低TNF-α及IL-6的分泌,所有结果显示两药联用优于单药组(p<0.05),柔性纳米脂质体组优于游离药物组(p<0.05)。结论 维甲酸倍他米松柔性纳米脂质体可以被角质形成细胞较好的摄取,具有良好的经皮渗透性,可以从抑制角质形成细胞的异常增殖及分泌TNF-α、IL-6等炎症因子起到协同治疗银屑病的作用。本研究补充了前期研究的不足,为维甲酸倍他米松柔性纳米脂质体的制剂开发提供了细胞学理论依据。

Abstract:

Objective To investigate the synergistic therapeutic effect of betamethasone retinoate flexible nano-liposome in psoriasis cell model, and to provide a theoretical basis for the development of retinoate betamethasone flexible nano-liposome. Methods Use ODA-FITC-labeled flexible nanoliposomes to investigate their cellular uptake and percutaneous penetration; use KGF to induce HaCat cells to establish a psoriasis abnormal proliferation cell model; use MTT method to investigate the flexibility of betamethasone retinoic acid with OD value as an indicator. The effect of nanoliposomes on the abnormal proliferation of cell models; enzyme-linked immunoassay was used to detect the changes of inflammatory factors. Results The uptake of flexible nanoliposomes on HaCaT cells is time-dependent and has good transdermal permeability. KGF stimulates