一测多评法同时测定魅力年华喷雾剂中7种有效成分的含量

Simultaneous Determination of 7 Effective Components in Meili Nianhua Spray by Quantitative Analysis of Multi-components by Single Marker

关键词:  一测多评法  气相色谱  魅力年华喷雾剂

Key words:  quantitative analysis of multi-components by single marker  GC  Meili Nianhua spray

摘要:

目的 利用气相色谱法和一测多评法建立同时测定魅力年华喷雾剂中石竹素、桉叶油醇、桂皮醛、芳樟醇、α-蒎烯、β-蒎烯和龙脑7种有效成分的含量，从而控制其质量的方法。方法 采用HP-5毛细管色谱柱（30 m×0.32 mm，0.25 μm），载气为氮气，氢火焰离子检测器的温度为240℃，程序升温，进样口温度240℃，进样量1 μL，分流比20：1。结果 石竹素、桉叶油醇、桂皮醛、芳樟醇、α-蒎烯、β-蒎烯、龙脑分别在0.020 0~0.140 0，0.360 0~2.520 0，0.205 0~1.435 0，0.012 5~0.087 5，0.070 0~0.490 0，0.128 0~0.896 0，0.060 0~0.420 0 mg·mL^-1内呈现良好的线性关系（r ≥ 0.999 6），平均含量分别为0.120 5，1.963 3，0.650 4，0.036 3，0.791 9，0.416 1，0.074 1 mg·mL^-1，与内标法相比，均无显著差异，平均加样回收率分别为102.03%，103.04%，102.36%，97.42%，99.52%，97.11%，102.40%，RSD分别为1.38%，0.61%，0.72%，1.32%，1.37%，0.99%。结论 该方法简捷、准确、重现性好，可用于魅力年华喷雾剂的质量控制。

Abstract:

OBJECTIVE To establish simultaneous determination of caryophylline, cineol, cinnamic aldehyde, linalool, α-pinene, β-pinene and borneol by gas chromatography and quantitative analysis of multi-components by single marker method, to control the quality of the 7 effective components in Meili Nianhua spray. METHODS HP-5 capillary column (30 m×0.32 mm, 0.25 μm) was used. The carrier gas was nitrogen. The temperature of hydrogen flame ionization detector was 240℃ and the injection temperature was 240℃. The injection volume was 1 μL and the shunt ratio was 20:1. RESULTS The calibration curves of caryophyllin, cineol, cinnamaldehyde, linalool, α-pinene, β-pinene and borneol were respectively in good linearity within the range of 0.020 0-0.140 0, 0.360 0-2.520 0, 0.205 0-1.435 0, 0.012 5-0.087 5, 0.070 0-0.490 0, 0.128 0-0.896 0, 0.060 0-0.420 0 mg·mL^-1(r ≥ 0.999 6), and the average contents were 0.120 5, 1.963 3, 0.650 4, 0.036 3, 0.791 9, 0.416 1 and 0.074 1 mg·mL^-1, respectively, with no significant difference compared with the internal standard method, and the corresponding average recoveries were 102.03%, 103.04%, 102.36%, 97.42%, 99.52%, 97.11% and 102.40%, RSDS were 1.38%, 0.61%, 0.72%, 1.32%, 1.37% and 0.99%, respectively. CONCLUSION The method is simple, accurate and reproducible, and can be used for the quality control of Meili Nianhua spray.