| 引用本文: | 宋京美,龚韬,杨薇,胡银燕,严文利,王夏,冯利.HPLC测定益肾骨康颗粒中莫诺苷、马钱苷、野黄芩苷、柚皮苷的含量[J].中国现代应用药学,2021,38(7):841-844. |
| SONG Jingmei,GONG Tao,YANG Wei,HU Yinyan,YAN Wenli,WANG Xia,FENG Li.Determination of Morroniside, Loganin, Scutellarin and Naringin in Yishen Gukang Granules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(7):841-844. |
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| HPLC测定益肾骨康颗粒中莫诺苷、马钱苷、野黄芩苷、柚皮苷的含量 |
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宋京美1, 龚韬1, 杨薇1, 胡银燕1, 严文利1, 王夏1, 冯利2
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1.北京市临床药学研究所, 北京 100035;2.国家癌症中心/国家肿瘤临床医学研究中心/中国医学科学院北京协和医学院肿瘤医院, 北京 100021
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| 摘要: |
| 目的 通过HPLC对益肾骨康颗粒中莫诺苷、马钱苷、野黄芩苷、柚皮苷的含量进行定量分析。方法 色谱柱为Kinetex EVO C18（150 mm×4.6 mm，5 μm），进样量为10 μL，流动相采用乙腈（A）和0.5%磷酸水溶液（B）进行梯度洗脱。检测波长设为240 nm （莫诺苷、马钱苷）、283 nm （柚皮苷）、335 nm （野黄芩苷）进行检测。结果 莫诺苷、马钱苷、野黄芩苷、柚皮苷标准曲线分别为Y=1.897 8×106X-22 664.29（r=0.999 9），Y=1.792 0×106X+3 040.43（r=0.999 9），Y=3.745 9×106X+3 287（r=0.999 9），Y=1.854 0×106X+654.57（r=0.999 8）；线性范围分别为0.363 5~2.544 5，0.238 2~1.667 6，0.087 1~0.609 4，0.078 3~0.548 2 μg，平均加样回收率分别为98.25%，96.35%，95.74%，95.76%，RSD分别为0.85%，0.92%，1.06%，0.67%。结论 该法操作简单，结果具有较好的仪器精密度、稳定性和重复性，可作为益肾骨康颗粒质量控制的有效方法，而不同批次益肾骨康颗粒有效成分含量存在不同程度差异。 |
| 关键词: 益肾骨康颗粒 莫诺苷 马钱苷 野黄芩苷 柚皮苷 高效液相色谱法 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.07.012 |
| 分类号:R917.101 |
| 基金项目:北京市科技计划项目（Z171100001717017）；中国医学科学院临床与转化基金项目（2019XK320072） |
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| Determination of Morroniside, Loganin, Scutellarin and Naringin in Yishen Gukang Granules by HPLC |
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SONG Jingmei1, GONG Tao1, YANG Wei1, HU Yinyan1, YAN Wenli1, WANG Xia1, FENG Li2
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1.Beijing Institute of Clinical Pharmacy, Beijing 100035, China;2.National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China
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| Abstract: |
| OBJECTIVE To determine the content of morroniside, loganin, scutellarin and naringin in Yishen Gukang granules by high performance liquid chromatography(HPLC). METHODS The HPLC method was performed on Kinetex EVO C18column(150 mm×4.6 mm, 5 μm). The gradient elution was adopted with the mobile phase consisting of acetonitrile(A) and 0.5% phosphoric acid aqueous(B), and the injection volume was 10 µL. The detection wavelengths were set at 240 nm (morroniside and loganin), 283 nm(naringin) and 335 nm(scutellarin). RESULTS The calibration curves of morroniside, loganin, scutellarin and naringin were Y=1.897 8×106X‒22 664.29(r=0.999 9), Y=1.792 0×106X+3 040.43(r=0.999 9), Y=3.745 9×106X+328 7(r=0.999 9), Y=1.854 0×106X+654.57(r=0.999 8) and the linear ranges were 0.363 5‒2.544 5, 0.238 2‒1.667 6, 0.087 1‒0.609 4, 0.078 3‒0.548 2 μg, respectively. The average recoveries of morroniside, loganin, scutellarin and naringin were 98.25%, 96.35%, 95.74%, 95.76% and the RSD were 0.85%, 0.92%, 1.06%, 0.67%, respectively. CONCLUSION The method is simple, accurate, stable and reproducible for the quality control of Yishen Gukang granules. The contents of active ingredients in different batches of Yishen Gukang granules are different. |
| Key words: Yishen Gukang granules morroniside loganin scutellarin naringin HPLC content determination |
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