槲皮素通过NLRP炎症小体相关通路对顺铂诱导肝损伤的保护作用

Protective Effect of Quercetin on Cisplatin-induced Liver Injury Through NLRP Inflammatory Body Related Pathway

关键词:  槲皮素  NLRP炎症小体  顺铂  肝损伤

Key words:  quercetin  NLRP inflammatory body  cisplatin  liver injury

摘要:

目的 探讨槲皮素通过NLRP炎症小体相关通路对顺铂诱导肝损伤的保护作用及其生物学机制。方法 在顺铂处理之前给小鼠灌胃槲皮素；通过血清生化分析和肝组织病理学分析检查小鼠的肝损伤情况；通过Western blotting检测凋亡相关蛋白和NLRP3炎症小体相关蛋白的变化。结果 槲皮素可通过抑制血清谷丙转氨酶和谷草转氨酶水平升高，恢复肝脏组织病理学改变，从而有效防治顺铂诱导的肝损伤。同时，槲皮素可以明显抑制氧化应激相关因子的产生，降低肝细胞凋亡，降低Bax、Bad、裂解的caspase-3和增加Bcl-2表达。此外，NLRP3炎性小体途径在槲皮素的作用下被灭活。结论 槲皮素可以抑制顺铂诱导的小鼠肝损伤，可能是通过调节NLRP3炎症小体相关途径来实现的。

Abstract:

OBJECTIVE To explore the protective effect and biological mechanism of quercetin on cisplatin-induced liver injury through the NLRP inflammatory body related pathway. METHODS Before cisplatin treatment, mice were chronically administered with quercetin; the liver injury of mice was examined by serum biochemical analysis and liver histopathological analysis; the changes of apoptosis-related protein and NLRP3 inflammatory body-related protein were detected by Western blotting. RESULTS Quercetin could effectively prevent levels of serum alanine aminotransferase and aspartate aminotransferase and restoring liver histopathological changes, thereby effectively preventing liver injury injected by cisplatin. At the same time, quercetin could significantly inhibit the production of oxidative stress-related factors, reduce hepatocyte apoptosis, reduce Bax, Bad, cleaved caspase-3 and increase Bcl-2 expression. In addition, the NLRP3 inflammatory corpuscle pathway was inactivated by quercetin. CONCLUSION Quercetin inhibits cisplatin-induced liver injury in mice, which may be related to regulating NLRP3 inflammatory body-related pathways.