| 引用本文: | 朱丽萍,王心睿.基于细胞模型的高通量筛选技术在药物研发中的应用进展[J].中国现代应用药学,2021,38(23):3050-3056. |
| ZHU Liping,WANG Xinrui.Advances in the Application of High-throughput Screening Techniques Based on Cell Models in Drug Development[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(23):3050-3056. |
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| 摘要: |
| 发现一种具有临床疗效的新药，通常需要对成千上万种化合物进行逐一筛选，获得目标化合物后，再对其进一步深入验证，这个过程工作量大且周期长。高通量高内涵筛选技术的出现缩短发现目标化合物的时间，选用适当的生物模型提高了目标化合物的验证结果的准确性。本文主要对干细胞生物模型、CRISPR/Cas9基因编辑细胞模型及3D细胞培养模型在高通量高内涵药物筛选中的应用作一综述，同时简述了这些方法在妇科肿瘤治疗药物研发中的应用。 |
| 关键词: 高通量 高内涵 干细胞 CRISPR/Cas9 3D细胞培养 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.23.023 |
| 分类号:R965.2 |
| 基金项目:福建省妇幼保健院科技创新启动基金项目（妇幼YCXB18-05；妇幼YCXM19-03） |
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| Advances in the Application of High-throughput Screening Techniques Based on Cell Models in Drug Development |
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ZHU Liping1,2, WANG Xinrui2,3
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1.Fujian Children's Hospital, Fuzhou 350011, China;2.Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou 350001, China;3.Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, National Health and Family Planning Commission, Fuzhou, 350013, China
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| Abstract: |
| The discovery of a new drug with clinical efficacy usually requires the screening of thousands of compounds one by one. When the target compound is obtained, it needs to be further verified. This process requires a lot of work and a long cycle. The emergence of high throughput and high content screening technology has shortened the time to find effective drugs, and the selection of appropriate biological models has improved the accuracy of screening results. This article mainly reviews the applications of stem cell biological models, CRISPR/Cas9 gene editing cell models, and 3D cell culture models in high throughput and high content drug screening, and briefly describes the application of these methods in the research and development of drugs for the treatment of women's malignant tumors. |
| Key words: high throughput screening high content screening stem cell CRISPR/Cas9 3D cell culture |
