GC-MS/MS测定人血浆中依替唑仑的浓度及其临床应用

Concentration Determination of Etizolam in Human Plasma by GC-MS/MS and Its Clinic Application

关键词:  依替唑仑  气相色谱-串联质谱法  血药浓度

Key words:  etizolam  GC-MS/MS  plasma drug concentration

摘要:

目的 建立人血浆中依替唑仑的GC-MS/MS检测方法，为临床药物分析提供依据。方法 血浆样品以甲苯提取后，采用色谱柱BR-5MS毛细管柱（30 m×0.25 mm，0.25 μm）程序升温，以多反应监测模式进行离子检测。利用保留时间和离子对比例定性，外标法和工作曲线定量，建立人血浆中依替唑仑的GC-MS/MS检测方法。结果 依替唑仑在0.5~600.0 ng·mL^–1内线性关系良好，相关系数r=0.995，定量下限为0.5 ng·mL^–1，三水平的萃取回收率和方法回收率为70%~110%，日内、日间精密度RSD均<10%，方法的稳定性良好，符合生物样品测定要求。结论 该方法灵敏准确，简便快速，适用于依替唑仑血药浓度检测。

Abstract:

OBJECTIVE To establish a GC-MS/MS method for the detection of etizolam in human plasma and provide the basis for clinical drug analysis. METHODS After extraction with toluene, plasma samples were subjected to column BR-5MS capillary column(30 m×0.25 mm, 0.25 μm) with temperature programming and ion detection in multiple reaction monitoring mode. The GC-MS/MS quantitative determination of etizolam was based on retention time and ratio of ions. The quantitative analysis was based on external standard method and calibration curve. RESULTS The linear range of etizolam was 0.5-600.0 ng·mL^-1, the coefficient of correlation r was 0.995, the lower limit of quantification was 0.5 ng·mL^-1, the recoveries ranged from 70% to 110% at three spiked levels, the RSD of inter-and intra-day was <10%, the stability of method was good, which met the requirements of biological sample determination. CONCLUSION The method is sensitive, accurate, simple and rapid, and suitable for the detection of etizolam plasma concentration.