HPLC-MS/MS同时测定银花芒果片中11种成分的含量

Simultaneous Determination of 11 Components in Yinhua Mangguo Tablets by HPLC-MS/MS

关键词:  高效液相-三重四极杆串联质谱法  银花芒果片  11种成分  含量测定

Key words:  HPLC-MS/MS  Yinhua Mangguo tablets  11 components  content determination

摘要:

目的 建立HPLC-MS/MS同时测定银花芒果片中绿原酸、灰毡毛忍冬皂苷乙、川续断皂苷乙、芒果苷、槲皮素、咖啡酸、木犀草苷、阿魏酸、桔梗皂苷D、橙皮苷和甘草苷含量的方法。方法 采用Agilent Porshell SB-C₁₈柱(4.6 mm×50 mm，2.7 μm)，以乙腈-0.1%甲酸水溶液为流动相，梯度洗脱，柱温30 ℃，流速0.5 mL·min^-1。质谱采用电喷雾离子源，以多反应监测模式进行定量分析。 结果 11种成分在一定浓度范围内与峰面积呈良好线性关系，相关系数r均>0.999；平均回收率(n=9)为98.15%~98.81%，RSD均≤0.6%；4批样品含量范围分别为4.97~5.24，8.51~9.28，2.97~3.23，6.31~6.68，0.26~0.30，0.80~0.93，0.22~0.25，0.33~0.38，0.95~1.07，4.08~4.36，1.93~2.21 mg·g^-1。结论 该方法简便、灵敏、准确、可靠，可用于银花芒果片的质量控制。

Abstract:

OBJECTIVE To establish a method for the simultaneous determination of chlorogenic acid, macranthoidin B, dipsacoside B, mangiferin, quercetin, caffeic acid, luteolin-7-O-glucoside, ferulic acid, platycodin D, hesperidin and liquiritinin in Yinhua Mangguo tablets by HPLC-MS/MS. METHODS The separation was performed on an Agilent Porshell SB-C₁₈ column(4.6 mm×50 mm, 2.7 μm). The mobile phase was consisited of acetonitrile-0.1% formic acid by gradient elution at 30 ℃ column temperature. The flow rate was 0.5 mL·min^-1. For quantitation, electrospray ionization source was applied and the mass spectrometer was operated in multiple reaction monitoring modes. RESULTS Eleven components showed good linearity with correlation coefficient >0.999. The average recoveries(n=9) ranged from 98.15% to 98.81% with RSD≤0.6%. The contents in 4 batches of samples were 4.97-5.24, 8.51-9.28, 2.97-3.23, 6.31-6.68, 0.26-0.30, 0.80-0.93, 0.22-0.25, 0.33-0.38, 0.95-1.07, 4.08-4.36, 1.93-2.21 mg·g^-1, respectively. CONCLUSION The method was simple, sensitive, accurate and reliable for the quality control of Yinhua Mangguo tablets.