| 引用本文: | 叶珂君,周小琳,欧贤红.儿茶酚胺检测技术的研究进展[J].中国现代应用药学,2022,39(2):253-260. |
| YE Kejun,ZHOU Xiaolin,OU Xianhong.Research Progress on Detection Technology of Catecholamines[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(2):253-260. |
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| 摘要: |
| 儿茶酚胺(catecholamines，CAs)是一类生物源性化合物，它们作为神经递质和激素在人和动物体内发挥着重要的生理作用。它们是诊断疾病和功能障碍的重要标志物，也是拟交感神经药物制剂的成分。因此生物样品、活细胞中CAs的测定方法，以及它们的精确度、灵敏度、选择性及实时性，对疾病的诊断及病理生理机制的研究具有重要意义。本文从临床生物样品、活体细胞、活体等不同层次综述近5年来CAs的检测方法，描述包括荧光成像法、电化学法、质谱法、光度分析法、非损伤成像技术等检测方法的性能特征及应用。 |
| 关键词: 儿茶酚胺 荧光成像法 质谱法 电化学法 非损伤成像技术 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.02.020 |
| 分类号:R914.1 |
| 基金项目:国家自然科学基金项目(81700308，31871181)；四川省心血管疾病防治协同创新中心专项资金项目(xtcx2016-19) |
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| Research Progress on Detection Technology of Catecholamines |
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YE Kejun, ZHOU Xiaolin, OU Xianhong
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Key Laboratory of Medical Electrophysiology of Ministry of Education, Medical Electrophysiological Key Laboratory of Sichuan Province, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, China
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| Abstract: |
| Catecholamines(CAs) are a class of biologically derived compounds that, as neurotransmitters and hormones, play important physiological roles in humans and animals. They are important markers for the diagnosis of disease and dysfunction and are also components of sympathomimetic drugs. Therefore, a sensitive, accurate, highly selective, and real-time catecholamine assay is of great significance for the diagnosis of disease and the study of its pathophysiological mechanism. In this paper, the detection methods of CAs in the past five years are reviewed from clinical biological samples, living cells and living bodies. The performance characteristics and applications of detection methods including fluorescence imaging, electrochemical method, mass spectrometry, photometric analysis and non-invasive imaging technology are described. |
| Key words: catecholamines fluorescence probe method mass spectrometry electrochemical method non-invasive imaging technology |
