| 引用本文: | 徐倩,赵亚,刘丽娟,吴亚运,赵瑞芝.醋柴胡不同极性洗脱部位对黄芩苷在小鼠体内分布的影响以及成分分析[J].中国现代应用药学,2021,38(8):912-918. |
| XU Qian,ZHAO Ya,LIU Lijuan,WU Yayun,ZHAO Ruizhi.Effects of Different Polar Elution Parts of Vinegar Bupleuri Radix on the Distribution of Baicalin in Mice and Its Component Analysis[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(8):912-918. |
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| 摘要: |
| 目的 考察醋柴胡不同极性洗脱部位对黄芩苷在小鼠体内分布的影响，并运用相关性分析初步探讨醋柴胡配伍黄芩苷在不同脏器中的增效活性成分。方法 通过多次给药，LC-MS/MS测定小鼠各组织中黄芩苷的含量，考察醋柴胡不同极性洗脱部位对黄芩苷在小鼠体内分布的影响；Q-Exactive Plus对醋柴胡不同极性部位的成分进行分析，SPSS 21.0软件进行相关性分析并探讨可能活性成分。结果 与黄芩苷单用组相比，联用组（95%乙醇洗脱部位）肝脏、肺脏、脾脏中黄芩苷浓度升高，75%乙醇洗脱部位肺脏中药物浓度升高，50%乙醇洗脱部位脾脏中药物浓度升高；成分相关性分析结果表明醋柴胡中21个化合物与黄芩苷在小鼠肝脏中分布呈显著性正相关，主要为醋制后产生的乙酰化皂苷；另13个化合物与黄芩苷在小鼠肺脏分布呈显著性相关，主要为生柴胡皂苷。结论 乙酰化皂苷、生柴胡皂苷可能分别是醋柴胡治疗肝脏、肺脏疾病的活性成分，其结果有待进一步验证。 |
| 关键词: 黄芩苷 醋柴胡 体内分布 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.08.003 |
| 分类号:R285.5 |
| 基金项目:国家自然科学基金项目(81573612)；广东省科技计划项目(2017A020211013) |
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| Effects of Different Polar Elution Parts of Vinegar Bupleuri Radix on the Distribution of Baicalin in Mice and Its Component Analysis |
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XU Qian, ZHAO Ya, LIU Lijuan, WU Yayun, ZHAO Ruizhi
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School of Second Clinical, Guangzhou University of Chinese Medicine/Guangdong Province Hospital of Traditional Chinese Medical, Guangzhou 510006, China
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| Abstract: |
| OBJECTIVE To investigate the effect of different polar elution parts of vinegar Bupleuri Radix on the distribution of baicalin in mice, and to preliminary explore its synergistic active components in different organs of vigenar Bupleuri Radix combined with baicalin by correlation analysis. METHODS Study on the effects of distribution of baicalin of different polar elution parts of vinegar Bupleuri Radix by multiple doses; the content of baicalin in each tissue was determined by LC-MS/MS; Q-Exactive Plus was used to analyze the components of different polar elution parts of vinegar Bupleuri Radix and the possible active components, and the correlation analysis was carried out by SPSS 21.0 software to explore the possible active components. RESULTS Compared with single group of baicalin, the combined group(95% ethanol elution part) increased the concentration of baicalin in liver, lung and spleen, 75% ethanol elution part increased the concentration of baicalin in lung, and 50% ethanol elution part increased the concentration of baicalin in spleen. Correlation analysis showed that 21 compounds in vinegar Bupleuri Radix were significantly positively correlated with the distribution of baicalin in liver, mainly acetylated saponins produced by vinegar preparation. The other 13 compounds showed significant correlation with the distribution of baicalin in the lung, most of them were raw saikosaponin. CONCLUSION Acetylated saponins, primary saponins may separately be the active components in the treatment of liver and lung, the results need to be further verified. |
| Key words: baicalin vinegar Bupleuri Radix distribution in vivo |
