共振光散射光谱法测定依地酸钙钠注射液的含量

Content Determination of Calcium Disodium Edetate Injections by Resonance Light Scattering Spectrometry

关键词:  依地酸钙钠  注射液  草酸钠  共振光散射法  含量测定

Key words:  calcium disodium edetate  injection  sodium oxalate  resonance light scattering method  content determination

摘要:

目的 建立一种快速测定依地酸钙钠注射液含量的方法。方法 采用共振光散射光谱技术测定0.01 mol·L^–1 NaOH的碱性条件下依地酸钙钠与草酸钠反应前后的共振光散射光谱强度差。结果 采用激发和发射光带宽5.0 nm、扫描速度2 000 nm·min^–1时，依地酸钙钠所解离的钙离子与草酸根形成草酸钙分子，从而使体系的共振光散射光谱强度减弱，在激发光与发射光波长均在273 nm处时，体系的共振光散射光谱强度差值最大，并在0.30~1.20 mg·L^–1内呈线性关系，相关系数R=0.999 2。本法检测限为0.005 8 mg·L^–1，定量限为0.019 3 mg·L^–1。结论 本法灵敏度高、专属性强、省时简便，可用于依地酸钙钠注射液的含量分析，测定值与容量分析法基本一致。

Abstract:

OBJECTIVE To establish a method to quickly determine the content of calcium disodium edetate injection. METHODS Resonance light scattering spectroscopy was used to determine the intensity difference of the resonance light scattering spectra before and after the reaction of calcium disodium edetate and sodium oxalate under the alkaline condition of 0.01 mol·L^-1NaOH. RESULTS When the excitation and emission bandwidth was 5.0 nm and the scanning speed was 2 000 nm·min^-1, the calcium ions dissociated by calcium disodium edetate and oxalate formed calcium oxalate molecules, which weaken the intensity of the resonance light scattering spectrum of the system. When the excitation and emission wavelengths were both at 273 nm, the difference in the intensity of the resonance light scattering spectrum of the system was the largest, and the relationship was linear in the range of 0.30-1.20 mg·L^-1, and the correlation coefficient R=0.999 2. The detection limit of this method was 0.005 8 mg·L^-1 and the limit of quantification was 0.019 3 mg·L^-1. CONCLUSION This method is highly sensitive, specific, time-saving and simple. It can be used for the content analysis of calcium disodium edetate injection. The measured value is basically consistent with the volumetric analysis method.