| 引用本文: | 刘学,刘欢欢,肖婷,周威,吴林菁,曲书阅,马莹,许乾丽,沈祥春,陶玲,茅向军.HPLC同时测定咳清胶囊中吗啡、磷酸可待因和岩白菜素含量[J].中国现代应用药学,2022,39(6):783-787. |
| LIU Xue,LIU Huanhuan,XIAO Ting,ZHOU Wei,WU Linjing,QU Shuyue,MA Ying,XU Qianli,SHEN Xiangchun,TAO Ling,MAO Xiangjun.Simultaneous Determination of Morphine,Codeine Phosphate and Bergenin in Keqing Capsules by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(6):783-787. |
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| HPLC同时测定咳清胶囊中吗啡、磷酸可待因和岩白菜素含量 |
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刘学1, 刘欢欢1, 肖婷1, 周威1, 吴林菁1, 曲书阅2, 马莹3, 许乾丽1,3, 沈祥春1, 陶玲1, 茅向军1,4
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1.贵州医科大学贵州省天然药物资源高效利用工程中心, 贵州省普通高等学校天然药物药理与成药性评价特色重点实验室, 贵州医科大学-贵阳市联合重点实验室, 天然药物资源优效利用重点实验室, 药学院, 贵阳 550025;2.贵州中医药大学, 贵阳 550025;3.贵州省食品药品检验所, 贵阳 550004;4.贵州省药品审评查验中心, 贵阳 550004
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| 摘要: |
| 目的 建立HPLC同时测定咳清胶囊中吗啡、磷酸可待因和岩白菜素含量的方法。方法 采用Ultimate®AQ-C18色谱柱(4.6 mm×250 mm,5 μm),乙腈(A)-0.1%磷酸溶液(B)为流动相,梯度洗脱,流速为1.0 mL·min-1,柱温为35℃,检测波长为210 nm,进样量10 μL。结果 吗啡、磷酸可待因和岩白菜素分别在20.018 2~320.291 2 μg·mL-1(r=0.999 9)、4.031 0~64.496 6 μg·mL-1(r=1.000 0)、19.760 0~316.160 0 μg·mL-1(r=1.000 0)内线性关系良好;平均回收率(n=6)分别为98.33%(RSD=2.72%),98.72%(RSD=2.77%)和98.54%(RSD=1.06%)。结论 该检测方法简便、准确、重复性好,可用于咳清胶囊中吗啡、磷酸可待因和岩白菜素3种有效成分的同时测定,也可为其质量控制提供参考。 |
| 关键词: 咳清胶囊 吗啡 磷酸可待因 岩白菜素 高效液相色谱法 含量测定 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.06.011 |
| 分类号:R284.1 |
| 基金项目:国家药典委员会2017年度药品标准提高工作(中药)(462);贵州省科技创新人才团队项目(黔科合人才团队[2015]4025号);贵州省高层次创新人才百层次人才项目(黔科合人才[2015]4029号);贵州省科技计划项目(黔科合平台人才[2017]5802) |
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| Simultaneous Determination of Morphine,Codeine Phosphate and Bergenin in Keqing Capsules by HPLC |
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LIU Xue1, LIU Huanhuan1, XIAO Ting1, ZHOU Wei1, WU Linjing1, QU Shuyue2, MA Ying3, XU Qianli1,3, SHEN Xiangchun1, TAO Ling1, MAO Xiangjun1,4
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1.Guizhou Provincial Engineering Center of Efficient Utilization of Natural Medicinal Resources, High Educational Key Laboratory of Natural Medicinal Pharmacology and Druggability of Guizhou Province, Joint Key Laboratory of Guizhou Medical University-Guiyang City, Key Laboratory of Optimal Utilization of Natural Medicinal Resources, School of Pharmaceutical Sciences, Guizhou Medical University, Guiyang 550025, China;2.Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China;3.Guizhou Provincial Food and Drug Inspection Institute, Guiyang 550004, China;4.Guizhou Drug Evaluation and Inspection Center, Guiyang 550004, China
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| Abstract: |
| OBJECTIVE To establish a HPLC method for the simultaneous determination of morphine, codeine phosphate and bergenin in Keqing capsules. METHODS Ultimate®AQ-C18(4.6 mm×250 mm, 5 μm) column was used; mobile phase was acetonitrile(A)-0.1% phosphoric acid(B) with gradient elution; flow rate was 1.0 mL·min–1; column temperature was 35 ℃; detection wavelength was 210 nm and the injection volume was 10 μL. RESULTS Good linear relationship was observed for morphine, codeine phosphate and bergenin in the range of 20.018 2-320.291 2 μg·mL–1 (r=0.999 9), 4.031 0-64.496 6 μg·mL–1 (r=1.000 0), and 19.760 0-316.160 0 μg·mL–1(r=1.000 0), respectively. The average recoveries(n=6) were 98.33%(RSD=2.72%), 98.72%(RSD=2.77%) and 98.54%(RSD=1.06%), respectively. CONCLUSION The established HPLC method is simple, accurate, repeatable and can be used for the determination of morphine, codeine phosphate and bergenin in Keqing capsule. It can also provide reference for its quality control. |
| Key words: Keqing capsules morphine codeine phosphate bergenin HPLC component determination |
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