GC-MS测定中药提取物中34种抑菌剂与抗氧剂

Determination of 34 Kinds of Antimicrobial Agents and Antioxidants in Chinese Herbal Extracts by GC-MS

关键词:  气相质谱法  中药提取物  抑菌剂  抗氧剂

Key words:  GC-MS  extracts of Chinese herbal  antibacterial agents  antioxidants

摘要:

目的 建立同时测定中药提取物中34种抑菌剂与抗氧剂的GC-MS分析方法。方法 采用Agilent DB-5MS毛细管色谱柱（60 m×320 μm，0.5 μm）；进样口温度：250℃；载气为高纯氦气；流速：2 mL·min^-1；进样量：1 μL；柱温40℃，保持1 min，以5℃·min^-1的速率升至300℃，保持10 min；电离源为电子轰击源（EI）；采用多反应监测模式进行定性及定量分析。结果 34种抑菌剂与抗氧剂在一定浓度范围内线性关系良好，相关系数r均>0.99，各成分在浸膏中回收率为83.7%~124.8%，RSD （n=6）为0.5%~5.2%；在粉末中的回收率为71.3%~119.6%，RSD （n=6）为0.7%~5.6%。结论 该方法的灵敏度、仪器精密度、回收率均能满足中药提取物中抑菌剂与抗氧剂检测的要求。

Abstract:

OBJECTIVE To establish a GC-MS method for simultaneous determination of 34 kinds of antimicrobial agents and antioxidants in Chinese herbal extracts. METHODS Using Agilent DB-5MS capillary column(60 m×320 μm, 0.5 μm); injection port temperature:250℃; carrier gas:high purity helium; flow rate:2 mL·min^-1; injection volume:1 μL; column temperature:40℃, maintained for 1 min, rose to 300℃ at a rate of 5℃·min^-1, maintained for 10 min; ionization source was EI source, the multi-reaction monitoring mode was used for qualitative and quantitative analysis. RESULTS The results showed that the linear range of 34 kinds of antimicrobial agents and antioxidants in a certain concentration range was great, and the correlation coefficients were all > 0.99. The recoveries of each component in the extractum ranged from 83.7% to 124.8%, and RSD(n=6) were between 0.5% and 5.2%. The recoveries in extract powder ranged from 71.3% to 119.6%, and the RSD(n=6) ranged from 0.7% to 5.6%. CONCLUSION The sensitivity, precision and recovery of the method can meet the instrument requirements of the detection of antibacterial agents and antioxidants in Chinese herbal extracts.