| 引用本文: | 代欣桃,李文锐,闻焜,杨志斌,杨自忠.颜氏大疣蛛中挥发油、氨基酸及微量元素分析[J].中国现代应用药学,2022,39(5):608-613. |
| DAI Xintao,LI Wenrui,WEN Kun,YANG Zhibin,YANG Zhizhong.Analysis of Volatile Oil, Amino Acids and Trace Elements in Macrothele Yani[J].Chin J Mod Appl Pharm(中国现代应用药学),2022,39(5):608-613. |
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| 颜氏大疣蛛中挥发油、氨基酸及微量元素分析 |
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代欣桃,李文锐,闻焜,杨志斌,杨自忠
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1.大理大学, 云南省昆虫生物医药研发重点实验室, 云南 大理 671000;2.大理大学, 中国西南药用昆虫及蛛形类资源开发利用 2011 协同创新中心, 云南 大理 671000;3.大理州食品药品检验所, 云南 大理 671000;4.大理大学, 药用特种昆虫开发国家地方联合工程研究中心, 云南 大理 671000
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| 摘要: |
| 目的 分析鉴定颜氏大疣蛛中的化学成分并测定含量。方法 采用水蒸气蒸馏法和索氏提取法提取挥发油，并用GC-MS进行分析鉴定；全自动氨基酸分析仪测定水解后氨基酸的含量；ICP-MS测定微量元素的含量。结果 水蒸气蒸馏法提取的挥发油共鉴定出22个化合物，索氏提取法18个化合物，共有化合物7个，其中油酸乙酯、亚油酸乙酯、十八酸乙酯含量较高；颜氏大疣蛛中含有16种氨基酸，包括7种人体必需氨基酸，氨基酸总量为10.53%，谷氨酸和精氨酸含量较高；富含11种人体必需微量元素，其中Zn、Fe、Mn含量较高，重金属元素As和Pb的含量符合限度要求，Cd和Cu的含量超出限度要求。结论 经鉴定2种方法提取的挥发油含量和成分具有一定的差异，其中一些成分具有一定的药用功效，此外，颜氏大疣蛛中还含有多种人体必需的氨基酸和微量元素。 |
| 关键词: 颜氏大疣蛛 挥发油 氨基酸 微量元素 气质联用 电感耦合电离子体质谱 |
| DOI：10.13748/j.cnki.issn1007-7693.2022.05.006 |
| 分类号:R284.1 |
| 基金项目:云南省科技计划项目（2016FA012）；云南省中药饮片产业发展专项（2019-YG-067）；云南省重大科技专项（生物医药）（202002AA100007）；大理州重点科技支撑专项计划（D2019NA01） |
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| Analysis of Volatile Oil, Amino Acids and Trace Elements in Macrothele Yani |
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DAI Xintao1,2,3,4, LI Wenrui1,2,3,4, WEN Kun4, YANG Zhibin1,2,3,5, YANG Zhizhong1,2,3,5
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1.Dali University, Yunnan Provincial Key Laboratory of Entomological Biopharmaceutical R&2.D, Dali 671000, China;3.Dali University, Yunnan Provincial 2011 Collaborative Innovation Center for Entomoceutics, Dali 671000, China;4.Dali Institute for Food and Drug Control, Dali 671000, China;5.Dali University, National-local Joint Engineering Research Center of Entomoceutics, Dali 671000, China
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| Abstract: |
| OBJECTIVE To separate and identify the chemical constituents in Macrothele yani and determine the content.METHODS The volatile oils were extracted by steam distillation method and Soxhlet extraction, and the volatile oils were identified by GC-MS. Total amino acids content was determined by amino acid autoanalyzer and trace elements content was determined by ICP-MS. RESULTS A total of 22 compounds were identified from the volatile oil extracted by steam distillation, while there were 18 kinds of volatile oils extracted by Soxhlet extraction. There were 7 volatile oils in common. Among them, ethyl oleate, ethyl linoleate and ethyl octadecanoate were the most abundant. Among those amino acids, Macrothele yani had 16 kinds of amino acids, including 7 kinds of essential amino acids. The total amount of amino acids was 10.53%. The contents of glutamic acid and arginine were relatively high. It was rich in 11 kinds of essential trace elements for human body, among which the content of Zn, Fe and Mn was higher, the content of heavy metal elements As and Pb met the limit requirements, and the contents of Cd and Cu exceeded the limit. CONCLUSION The volatile oil extracted from Macrothele yani by the two methods has great differences in type and content, some of them have certain medicinal effects. In addition, there are many kinds of necessary amino acids and trace elements for human in Macrothele yani. |
| Key words: Macrothele yani volatile oils amino acids trace elements GC-MS ICP-MS |
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