一测多评法测定不同产地漏芦中5种有效成分的含量

Determination of the Contents of Five Active Ingredients in Rhapontici Radix from Different Origins by Quantitative Analysis of Multi-components by Single Marker Method

关键词:  漏芦  一测多评  相对校正因子  含量测定

Key words:  Rhapontici Radix  quantitative analysis of multi-components by single marker  relative correction factor  content determination

摘要:

目的 采用一测多评法(quantitative analysis of multi-components by single marker,QAMS)测定漏芦中5种成分含量。方法 采用HPLC,流动相为乙腈(A)-0.02%磷酸水溶液(B),梯度洗脱;流速:1.0 mL·min^-1;柱温:30℃;检测波长:210 nm。以β-蜕皮甾酮为内参物,分别建立咖啡酸、甘草苷、迷迭香酸、齐墩果酸的相对校准因子,利用该相对校准因子计算其他4种成分含量。结果 咖啡酸、β-蜕皮甾酮、甘草苷、迷迭香酸、齐墩果酸分别在2.09~104.5,2.62~131.2,1.31~65.4,3.06~152.8,1.33~66.6μg·mL^-1内线性关系良好(r≥0.999 5),平均加样回收率分别为98.68%,99.21%,96.50%,97.44%,100.76%,RSD分别为1.12%,0.95%,1.63%,1.60%,2.03%。12批不同产地的漏芦中5种有效成分采用QAMS与外标法的实测值之间无显著性差异。结论 该方法结果准确可靠、重复性好,可用于漏芦的质量控制。

Abstract:

OBJECTIVE To establish quantitative analysis of multi-components by single-marker(QAMS) for determining contents of five ingredients from Rhapontici Radix.METHODS The HPLC method was established and the mobile phase consisted of acetonitrile (A)-0.02%phosphoric acid(B) as mobile phase in a gradient mode.The flow rate was 1.0 mL·min^-1,column temperature was set at 30℃and detection wavelength was 210 nm.Usingβ-ecdysterone as an internal reference,the relative calibration factors of caffeic acid,liquiritin,rosmarinic acid and oleanolic acid were established respectively,and the contents of the other four components were calculated using the relative calibration factors.RESULTS Caffeic acid,β-ecdysterone,liquiritin,rosmarinic acid and oleanolic acid showed good linear relationships within the ranges of 2.09-104.5,2.62-131.2,1.31-65.4,3.06-152.8,1.33-66.6μg·mL^-1(r ≥ 0.999 5),whose average recoveries were 98.68%,99.21%,96.50%,97.44%,100.76%,with the RSDs of 1.12%,0.95%,1.63%,1.60%,2.03%,respectively.The contents of five active ingredients in 12 batches of Rhapontici Radix from different origins determined by QAMS had no significantly different with external standard method.CONCLUSION The method is accurate,reliable and repeatable,which can be used for the quality control of Rhapontici Radix.