苁蓉舒痉颗粒HPLC-PDA全波长最大值特征图谱研究

Study on HPLC-PDA Full-wavelength Maximum Characteristic Chromatogram of Congrong Shujing Granules

关键词:  苁蓉舒痉颗粒  HPLC-PDA全波长-最大值法  特征图谱

Key words:  Congrong Shujing Granules  HPLC-PDA full-wavelength maximum method  characteristic chromatogram

摘要:

目的 探究HPLC-PDA全波长最大值法建立苁蓉舒痉颗粒特征图谱，为评价其整体质量提供依据。方法 采用HPLC-PDA 210~800 nm全波长最大值法检测，Agilent Zorbax Eclipse XDB-C₁₈色谱柱（250 mm×4.6 mm，5 μm），乙腈（A）-0.1%磷酸溶液（B）为流动相，梯度洗脱，流速为1.0 mL·min^-1，柱温为25℃，进样量为20 μL，洗脱时间为60 min，PDA最大值显示谱图。结果 建立了以丹酚酸B为参照峰的苁蓉舒痉颗粒HPLC-PDA全波长最大值法特征图谱共有模式，确定了20个共有特征峰并归属标定其中6个特征峰；分析的10批苁蓉舒痉颗粒与共有模式之间具有良好的相似性，相似度均>0.950。结论 首次建立的HPLC-PDA全波长最大值法特征图谱，重复性好，灵敏度高，可更精准地提取并表征苁蓉舒痉颗粒效应组分群的信息，为苁蓉舒痉颗粒质量标准的制订奠定了基础，为复杂体系的中药复方制剂特征图谱的建立提供了一种可供借鉴的新方法。

Abstract:

OBJECTIVE To explore the HPLC-PDA full-wavelength maximum method and to establish characteristic chromatogram of Congrong Shujing Granules, so as to provide a basis for evaluating its overall quality. METHODS The HPLC-PDA 210-800 nm full-wavelength maximum method was used to detect. Agilent Zorbax Eclipse XDB-C₁₈ column (250 mm×4.6 mm, 5 μm) was used as the stationary phase, and acetonitrile(A)-0.1% phosphoric acid solution(B) was used as the mobile phase. Gradient elution was performed, and PDA maximum value graph showed that the flow rate was 1.0 mL·min^-1, the column temperature was 25℃, the injection volume was 20 μL, and the elution time was 60 min. RESULTS The common mode of the HPLC-PDA full-wavelength maximum characteristic chromatogram of Congrong Shujing Granules with salvianolic acid B as the reference peak was established, and 20 common characteristic peaks were identified and assigned to 6 of them. The analyzed 10 batches of Congrong Shujing Granules had a good similarity with the common pattern, and the similarity was all >0.950. CONCLUSION The first established HPLC-PDA full-wavelength maximum method characteristic chromatogram has good reproducibility and high sensitivity. It can more accurately characterize and extract the information of the medicinal component group of Congrong Shujing Granules, and lay the foundation for formulating the quality standard of Congrong Shujing Granules. It provides a new method that can be used for reference for the establishment of the characteristic chromatogram of Chinese herbal compound preparations with complex systems.