基于肠道微生态探讨黄芪超微粉饮片的抗疲劳作用

Study on Anti-fatigue effect of Superfine Powder of Astragali radix Based on the intestinal microecology

关键词:  黄芪  超微粉  抗疲劳  16S rDNA  肠道菌群  微生物多样性

Key words:  Astragali Radix  superfine powder  anti-fatigue  16S rDNA  intestinal flora  micro-biodiversity

摘要:

目的 观察黄芪超微粉饮片对C57BL/6小鼠的抗疲劳作用及肠道菌群变化。方法 将C57BL/6小鼠分为黄芪超微粉高剂量组(2.50^-1)、中剂量组(1.25^-1)、低剂量组(0.63^-1)、传统饮片组(2.5^-1)、传统粉末组(2.5^-1)和空白组。连续灌胃给药30d，期间和给药后测定小鼠体质量、行为学指标和生化指标。在灌喂第30天时无菌取结肠内容物，提取DNA，扩增肠道菌群16S rDNA基因的V3+V4区，采用高通量测序分析β多样性和α多样性、分析门水平和属水平的物种组。结果 与空白组比较，黄芪超微粉饮片高剂量组小鼠的爬杆时间、转棒距离与跑步距离均延长，分别为14.8%(P<0.05)，26.8%(P<0.01)和16.2%(P<0.01)；小鼠肝糖原和骨骼肌ATP均升高，分别为20.48%和20.86%(P<0.01)。血清中的BUN和LA的含量下降了5.87%(P<0.01)和12.34%(P<0.01)；血清中T-SOD活性升高18.15%(P<0.01)、MDA水平显著降低16.14%(P<0.01)。小鼠肠道菌群属水平含量排在前20的为Ruminiclostridium(p=0.0017)和Lachnoclostridium(p=0.0141)，含量均增加。结论 黄芪超微粉具有明显的抗疲劳作用，比其他2种给药方式好，与增加能量供应、抗氧化、减少代谢物质蓄积直接相关。另外，黄芪超微粉组的肠道菌群与空白组有明显差异，肠道菌群变化或许与黄芪超微粉饮片抗疲劳间接相关。

Abstract:

OBJECTIVE To observe the anti-fatigue effect of Astragali Radix superfine powder on C57BL/6 mice and the changes of intestinal flora.METHODS The C57BL/6 mice were divided into Astragali Radix superfine powder high-dose group(2.50 g·kg^-1), medium-dose group(1.25 g·kg^-1), low-dose group(0.63 g·kg^-1), and traditional decoction group(2.5 g·kg^-1), traditional powder group(2.5 g·kg^-1) and blank group. Gavage was given for 30 consecutive days, during and after the administration, the body weight, behavioral indicators and biochemical indicators of the mice were measured. On the 30th day of perfusion, the contents of the colon were aseptically taken, DNA was extracted, the V3+V4 region of the 16S rDNA gene of the intestinal flora was amplified, and the β diversity and α diversity, the phylum and species of intestinal flora were analyzed by high-throughput sequencing.RESULTS Compared with the blank group, the rod climbing time, rotating rod distance and running distance of mice in the Astragali Radix superfine powder high-dose group were prolonged by 14.8%(P<0.05), 26.8%(P<0.01) and 16.2%(P<0.01); mouse liver glycogen and skeletal muscle ATP increased, respectively 20.48% and 20.86%(P<0.01) and the content of BUN and LA decreased by 5.87%(P<0.01) and 12.34%(P<0.01). The activity of T-SOD in serum increased by 18.15%(P<0.01), and the level of MDA was significantly reduced by 16.14%(P<0.01). The top 20 mouse gut microbiota levels were Ruminiclostridium(p=0.001 7) and Lachnoclostridium(p=0.014 1), both increased.CONCLUSION Astragali Radix superfine powder has obvious anti-fatigue effect, which is better than the other two methods of administration, and is directly related to increasing energy supply, anti-oxidation, and reducing the accumulation of metabolites. In addition, the intestinal flora of the Astragali Radix superfine powder group is significantly different from that of the blank group. The change of the intestinal flora may be indirectly related to the anti-fatigue of Astragali Radix superfine powder decoction pieces.