| 引用本文: | 孔伟浩,徐依桐,徐达峰,刘开科,张锦原,高珣.靶向垂钓技术在中药活性成分筛选中的研究进展[J].中国现代应用药学,2021,38(18):2288-2295. |
| KONG Weihao,XU Yitong,XU Dafeng,LIU Kaike,ZHANG Jinyuan,GAO Xun.Research progress of target fishing technology in screening active ingredients of traditional Chinese medicine[J].Chin J Mod Appl Pharm(中国现代应用药学),2021,38(18):2288-2295. |
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| 摘要: |
| 目的 研究近年来靶向垂钓技术（主要为配体垂钓和靶标垂钓）在中药活性成分筛选方面的进展，并做相关总结。方法 以近年来发表的相关文献为依据，对靶向垂钓-液质联用技术在中药活性成分的筛分与鉴定进行了综述。结果 目前常使用靶向垂钓技术筛分不同种类的中药活性成分，该方法具有筛分速度快、设备简单、操作简单及特异性好等优点。结论 对复杂多样的中药混合物进行分析时，利用靶向垂钓-液质联用技术可以较好地筛分目标物并鉴定其结构。 |
| 关键词: 中药 活性成分 靶标垂钓 配体垂钓 |
| DOI：10.13748/j.cnki.issn1007-7693.2021.18.016 |
| 分类号:R284.2 |
| 基金项目:国家自然科学基金项目（82104349） |
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| Research progress of target fishing technology in screening active ingredients of traditional Chinese medicine |
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KONG Weihao, XU Yitong, XU Dafeng, LIU Kaike, ZHANG Jinyuan, GAO Xun
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Jiangsu Ocean University, Lianyungang 222005, china
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| Abstract: |
| OBJECTIVE to study the progress of target fishing(mainly ligand fishing and target fishing) in the screening of active components in Chinese medicine and make the related summary. METHODS based on the related articles published in recent years, the screening and identification of active components in Chinese medicine by target fishing-liquid chromatography-mass spectrometry were reviewed. RESULTS Target fishing was often used to screen different kinds of active ingredients in chinese medicine. this method had the advantages of fast screening, simple equipment, simple operation and good specificity. CONCLUSION target fishing-liquid chromatography-mass spectrometry can be used to screen the target substances and identify their structures in the analysis of complex and diverse mixtures of chinese medicine. |
| Key words: traditional Chinese medicine active ingredient target fishing ligand fishing |
