HPLC同时测定杠板归及其蜜炙品中5个黄酮类成分含量

Simultaneous Determination of Five flavones in Polygonum perfoliatum L. and Its honey processed products by HPLC

关键词:  杠板归  蜜炙杠板归  含量测定  高效液相色谱法  质量控制

Key words:  polygonum perfoliatumL.  honey polygonum perfoliatum L.  content determination  HPLC  quality control

摘要:

目的 建立杠板归药材及其蜜炙品中5个活性成分（金丝桃苷、山奈酚-3-O-芸香糖苷、槲皮素、山奈素、异鼠李素）的HPLC含量测定方法。方法 采用phenomonex^®-C₁₈色谱柱（4.6 mm×250 mm，5 μm），以甲醇（A）-0.4%的磷酸溶液（B）为流动相进行线性梯度洗脱，流速1.0 mL·min^–1，柱温30℃，检测波长为360 nm。结果 金丝桃苷、山奈酚-3-O-芸香糖苷、槲皮素、山奈素和异鼠李素分别在浓度3.406~340.6，0.828~82.8，5.025~502.5，1.036~103.6，1.162~116.2 μg·mL^–1内与峰面积呈良好的线性关系（r≥0.999 3），平均加样回收率分别为100.04%，99.67%，97.96%，102.50%和98.99%。杠板归蜜炙后，金丝桃苷和山奈酚-3-O-芸香糖苷含量明显降低、槲皮素、山奈素、异鼠李素的含量明显升高。结论 该方法简便高效、专属性强，可为杠板归药材及其蜜炙品的质量控制和评价方法的建立提供依据。

Abstract:

OBJECTIVE to establish an HPLC method for the determination of hyperoside, kaempferol-3-o-rutin, quercetin, kaempferol and isorhamnetin in Polygonum perfoliatum L. and its honey processed products. METHODS The analysis was performed on Phenomonex^®-C₁₈ column(250 mm×4.6 mm, 5 μm) with the gradient elution of methanol(A)-0.4% phosphoric acid(B) solution at flow rate of 1.0 ml·min^-1. The column temperature was 30℃ and the detection wavelength was set at 360 nm. RESULTS The linear ranges of hyperoside, kaempferol-3-o-rutin, quercetin, kaempferol and isorhamnetin were 3.406-340.6, 0.828-82.8, 5.025-502.5, 1.036-103.6, 1.162-116.2 μg·mL^-1, respectively (r ≥ 0.999 3), the average recoveries of five components were 100.04%, 99.67%, 97.96%, 102.50% and 98.99%, respectively. After honey precessed, contents of hyperoside and kaempferol-3-O-rutoside in Polygonum perfoliatum decreased significantly, while the contents of quercetin, kaempferol and isorhamnetin increased significantly. CONCLUSION The method is simple, efficient, accurate and reliable, which can provide scientific basis for the quality control of the Polygonum perfoliatum L. and its honey processed products.