基于HPLC指纹图谱和化学计量学的不同产地雪菊质量评价

Quality evaluation of Coreopsis tinctorial from different producing areas based on HPLC fingerprint and chemometrics

关键词:  雪菊  指纹图谱  化学计量学  含量测定  质量评价

Key words:  coreopsis tinctorial  fingerprint  chemometrics  content determination  quality evaluation

摘要:

目的 建立雪菊HPLC指纹图谱，结合化学计量学，测定4种成分含量，评价不同产地雪菊质量。方法 采用依利特SinoChrom ODS-BP C₁₈色谱柱（4.6 mm×250 mm，5 μm），以乙腈-0.1%磷酸溶液为流动相，梯度洗脱；流速为0.6 mL·min^-1；检测波长为295 nm；柱温为30℃；建立不同产地雪菊指纹图谱，进行相似度评价、聚类分析、主成分分析、偏最小二乘法-判别分析，结合变量重要性投影值筛选指认主要差异成分，进行含量测定。结果 12批雪菊指纹图谱含21个共有峰，所有样品与对照图谱相似度均≥0.942；经聚类分析和主成分分析，12批雪菊聚为3类；偏最小二乘法-判别分析结果与聚类分析一致，变量重要性投影值筛选的4个主要差异成分含量分别为马里苷40.05~61.25 mg·g^–1，黄诺马苷7.44~19.82 mg·g^–1，芦丁0.85~2.03 mg·g^–1，绿原酸2.56~9.73 mg·g^–1。结论 建立的指纹图谱分析与含量测定方法可靠、重复性好，为雪菊的整体质量评价提供了参考。

Abstract:

OBJECTIVE To establish HPLC fingerprint of Coreopsis tinctorial, so as to evaluate the quality of Coreopsis tinctorial from different areas by content determination of four components combined with chemometrics. METHODS Elite SinoChrom ODS-BP C₁₈ column(4.6 mm×250 mm, 5 μm) was used, the mobile phase was acetonitrile-0.1% phosphoric acid solution, gradient elution, the flow rate was 0.6 mL·min^-1, the detection wavelength was 295 nm, and the column temperature was 30℃. The fingerprint of Coreopsis tinctorial from different producing areas was established, and the similarity evaluation, cluster analysis(CA), principal component analysis(PCA) and partial least square discriminant analysis(PLS-DA) were carried out. Combined with the projectionvalue of variables(VIP), the main difference components were identified and the content was determined. RESULTS The fingerprints of 12 batches of Coreopsis tinctorial contained 21 common peaks, and the similarity between all samples and the control map was ≥ 0.942. By CA analysis and PCA analysis, 12 batches of Coreopsis tinctorial were grouped into 3 groups. The results of PLS-DA analysis were consistent with those of CA analysis. The contents of four main differential components selected by VIP value were marinoside 40.05-61.25 mg·g^-1, flavanomanthin 7.44-19.82 mg·g^-1, rutin 0.85-2.03 mg·g^-1, chlorogenic acid 2.56-9.73 mg·g^-1, respectively. CONCLUSION The fingerprint analysis and content determination method established in this experiment is reliable and reproducible, which provides a basis for the overall quality evaluation of Coreopsis tinctorial.